EMAGE mouse gene expression spatial database

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EMAGE:4728

Cbs cystathionine beta-synthase ( MGI:88285)
TS17 (9.5 dpc)
in situ hybridisation

Data Images


Fig2A. from J Histochem Cytochem 51(3): 363-71, Robert K; Vialard F; Thiery E; Toyama K; Sinet PM; Janel N; London J, Expression of the cystathionine beta synthase (CBS) gene during mouse development and immunolocalization in adult brain. Copyright 2003 [PMID:12588964]

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Editors Note: The authors state that "In Fig 2A the purple spot at the base of the neck was an artifact".

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

4728_wholemount_moderate_3D_1.wlz

4728_wholemount_weak_3D_1.wlz

4728_wholemount_notDetected_3D_1.wlz

4728_wholemount_strong_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:4728_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

neural tube

detected

graded

At E9.5, the entire neural tube was labeled (Fig 2A), with the strongest labeling in the neural tube closure region.

embryo

detected

homogeneous

The authors state that expression is ubiquitous between E9.5 and E10.5

limb

strong

Strongest expression is in the limb buds, tail, and brain vesicles at E9.5-E10.5

tail

strong

Strongest expression is in the limb buds, tail, and brain vesicles at E9.5-E10.5

brain

strong

Strongest expression is in the limb buds, tail, and brain vesicles at E9.5-E10.5

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Cbs probeA
Entity Detected:	Cbs, cystathionine beta-synthase ( MGI:88285)
Notes:	The Cbs probe used in this study for whole-mount in situ hybridisations by Robert et al., 2003 [PMID:12588964] is stated as being "synthesized from previously described linearized template". Editors Note: This most probably refers to the description of the probe the authors used on sections, which is described as follows: "single-stranded RNA was synthesized from the NotI fragment of pBluescript, which contains the rat Cbs cDNA fragment (nucleotides 652-945), using T3 RNA polymerase as an antisense riboprobe.", and "A 350-bp cDNA fragment containing exons 5-8 of the Cbs gene was isolated from the rat Cbs orthologue (Swaroop et al. 1992 [PMID:1597473] ) ". The sequence of the rat (type 1 cDNA isoform) is shown in Fig 2 of Swaroop et al. and the accompanying M88344.1 GenBank entry, however the nucleotide numbering is different between the two and no NotI sites are found in these sequences. It is unclear from the published description which region the probe covers.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	9.5 dpc
Theiler Stage:	TS17
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Fixation:

4% paraformaldehyde

General Information

Authors:	Robert K; Vialard F; Thiery E; Toyama K; Sinet PM; Janel N; London J, 2003 [PMID:12588964] , Indexed and Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1177/002215540305100311] [ PMID:12588964] Robert K, Vialard F, Thiery E, Toyama K, Sinet PM, Janel N, London J 2003 Expression of the cystathionine beta synthase (CBS) gene during mouse development and immunolocalization in adult brain. J Histochem Cytochem (51):363-71
	[ PMID:1597473] Swaroop M, Bradley K, Ohura T, Tahara T, Roper MD, Rosenberg LE, Kraus JP 1992 Rat cystathionine beta-synthase. Gene organization and alternative splicing. J Biol Chem (267):11455-61
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source