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EMAGE:4736

Cdc25b cell division cycle 25 homolog B (S. pombe) ( MGI:99701)
TS15 (9 dpc)
in situ hybridisation

Data Images
EMAGE:4736
Fig4A. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2006.02.035] Dev Biol 294(1): 133-47, Benazeraf B; Chen Q; Peco E; Lobjois V; Medevielle F; Ducommun B; Pituello F, Identification of an unexpected link between the Shh pathway and a G2/M regulator, the phosphatase CDC25B. Copyright 2006. [PMID:16564519]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: The sections were taken form the levels indicated by the lines. The arrow indicates scattered cells displaying a higher level of staining.
Expression Pattern Description
Spatial Annotation:
EMAGE:4736Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4736_wholemount_moderate_3D_1.wlz
4736_wholemount_weak_3D_1.wlz
4736_wholemount_notDetected_3D_1.wlz
4736_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4736_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
future spinal cord
detected detected
CDC25B transcripts are present in the developing spinal cord.
neural tube
detected detected
regionalThe transcript was present in the entire neural tube, however there is no ventro-dorsal gradient of expression as seen in chicken. There are also scattered cells expressing a higher level of transcripts both in the ventral and dorsal neural tube.
caudal neuropore
not detected not detected
homogeneousCDC25B is absent from the caudal neuroepithelium.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Cdc25b probeA
Entity Detected:Cdc25b, cell division cycle 25 homolog B (S. pombe) ( MGI:99701)
Sequence:sense strand is shown

>Cdc25b probeA
ACTCCTGTCGAAAGGGCTTCTGAAGAAGATGACGGATTTGTGGACATCCTGGAGAGTGATTTAAAGGATG
ACGAGAAGGTCCCCGCGGGCATGGAGAACCTCATTAGTGCCCCACTGGTCAAAAAGCTGGATAAGGAAGA
GGAACAGGATCTCATCATGTTCAGCAAGTGCCAGAGGCTCTTCCGCTCCCCATCCATGCCATGCAGTGTG
ATCCGACCCATCCTCAAGAGGCTAGAGCGGCCCCAGGACCGGGATGTGCCTGTCCAGAGCAAGCGCAGGA
AAAGTGTGACACCCCTGGAAGAGCAGCAGCTTGAAGAACCTAAGGCCCGTGTCTTTCGCTCAAAGTCGCT
GTGTCATGAGATTGAGAACATCCTGGATAGTGACCACCGTGGACTGATCGGAGATTACTCTAAGGCCTTC
CTCCTGCAGACCGTGGATGGCAAACACCAAGACCTTAAGTACATCTCACCAGAAACTATGGTGGCCCTGT
TAACAGGCAAGTTCAGCAACATCGTGGAGAAATTTGTCATTGTGGACTGCAGATACCCCTATGAGTATGA
AGGCGGGCATATCAAGAATGCTGTGAACCTGCCCCTGGAACGGGATGCTGAGACCTTTCTGC
nt 999 - nt 1620 of NM_023117.2
Notes:The Cdc25b probe template used in this study by Benazeraf et al., 2006 [PMID:16564519] is described as follows: "A fragment of mouse CDC25B coding regions was obtained by PCR. The primers used were: 5'-ACTCCTGTCGAAAGGGCTTC-3' and 5'-GCAGAAAGGTCTCAGCATCC-3'. "
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:9 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Benazeraf B; Chen Q; Peco E; Lobjois V; Medevielle F; Ducommun B; Pituello F, 2006 [PMID:16564519] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.ydbio.2006.02.035] [ PMID:16564519] Banazaraf B, Chen Q, Peco E, Lobjois V, Medevielle F, Ducommun B, Pituello F 2006 Identification of an unexpected link between the Shh pathway and a G2/M regulator, the phosphatase CDC25B. Dev Biol (2006):133-47
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE