Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:4737

Cdc25b cell division cycle 25 homolog B (S. pombe) ( MGI:99701)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4737
Fig4B. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2006.02.035] Dev Biol 294(1): 133-47, Benazeraf B; Chen Q; Peco E; Lobjois V; Medevielle F; Ducommun B; Pituello F, Identification of an unexpected link between the Shh pathway and a G2/M regulator, the phosphatase CDC25B. Copyright 2006. [PMID:16564519]

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: antero-posterior axis of the limb bud is as indicated by the double arrow. Level of section is indicated by the line. arrow indicates expression in a discrete domain flanking the floor plate.
Expression Pattern Description
Spatial Annotation:
EMAGE:4737Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4737_wholemount_moderate_3D_1.wlz
4737_wholemount_possible_3D_1.wlz
4737_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4737_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
neural tube
detected detected
limb
detected detected
regionalCDC25B is expressed in the posterior part of the limb bud.
future spinal cord
strong strong
regionalCDC25B is strongly expressed in the dorsal spinal cord and in a discrete domain flanking the floor plate.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Cdc25b probeA
Entity Detected:Cdc25b, cell division cycle 25 homolog B (S. pombe) ( MGI:99701)
Sequence:sense strand is shown

>Cdc25b probeA
ACTCCTGTCGAAAGGGCTTCTGAAGAAGATGACGGATTTGTGGACATCCTGGAGAGTGATTTAAAGGATG
ACGAGAAGGTCCCCGCGGGCATGGAGAACCTCATTAGTGCCCCACTGGTCAAAAAGCTGGATAAGGAAGA
GGAACAGGATCTCATCATGTTCAGCAAGTGCCAGAGGCTCTTCCGCTCCCCATCCATGCCATGCAGTGTG
ATCCGACCCATCCTCAAGAGGCTAGAGCGGCCCCAGGACCGGGATGTGCCTGTCCAGAGCAAGCGCAGGA
AAAGTGTGACACCCCTGGAAGAGCAGCAGCTTGAAGAACCTAAGGCCCGTGTCTTTCGCTCAAAGTCGCT
GTGTCATGAGATTGAGAACATCCTGGATAGTGACCACCGTGGACTGATCGGAGATTACTCTAAGGCCTTC
CTCCTGCAGACCGTGGATGGCAAACACCAAGACCTTAAGTACATCTCACCAGAAACTATGGTGGCCCTGT
TAACAGGCAAGTTCAGCAACATCGTGGAGAAATTTGTCATTGTGGACTGCAGATACCCCTATGAGTATGA
AGGCGGGCATATCAAGAATGCTGTGAACCTGCCCCTGGAACGGGATGCTGAGACCTTTCTGC
nt 999 - nt 1620 of NM_023117.2
Notes:The Cdc25b probe template used in this study by Benazeraf et al., 2006 [PMID:16564519] is described as follows: "A fragment of mouse CDC25B coding regions was obtained by PCR. The primers used were: 5'-ACTCCTGTCGAAAGGGCTTC-3' and 5'-GCAGAAAGGTCTCAGCATCC-3'. "
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Benazeraf B; Chen Q; Peco E; Lobjois V; Medevielle F; Ducommun B; Pituello F, 2006 [PMID:16564519] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.ydbio.2006.02.035] [ PMID:16564519] Banazaraf B, Chen Q, Peco E, Lobjois V, Medevielle F, Ducommun B, Pituello F 2006 Identification of an unexpected link between the Shh pathway and a G2/M regulator, the phosphatase CDC25B. Dev Biol (2006):133-47
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE