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EMAGE:4744

Nr6a1 nuclear receptor subfamily 6, group A, member 1 ( MGI:1352459)
TS15 (25 Somite no.)
in situ hybridisation

Data Images
EMAGE:4744
Figure 1D. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2005.12.017] Dev Biol 293: 13-24, Chung AC; Xu X; Niederreither KA; Cooney AJ, "Loss of orphan nuclear receptor GCNF function disrupts forebrain development and the establishment of the isthmic organizer." Copyright 2006 [PMID:16530751]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4744Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4744_wholemount_moderate_3D_1.wlz
4744_wholemount_notDetected_3D_1.wlz
4744_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4744_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future forebrain
weak weak
GCNF expression was markedly reduced in the forebrain and midbrain regions, compared to earlier stages
future midbrain
weak weak
GCNF expression was markedly reduced in the forebrain and midbrain regions, compared to earlier stages
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Nr6a1 probeA
Entity Detected:Nr6a1, nuclear receptor subfamily 6, group A, member 1 ( MGI:1352459)
Notes:The Nr6a1 (GCNF) probe used in this study by Chung et al., 2006 [PMID:16530751] is of unreported sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:129/Sv * C57BL/6
Age:25 Somite no.
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:MEMFA (0.2 M MOPS, pH7.4, 2 mM EGTA, 1 mM MgSO4, 3.7% formaldehyde)
General Information
Authors:Chung AC; Xu X; Niederreither KA; Cooney AJ, 2006 [PMID:16530751] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.ydbio.2005.12.017] [ PMID:16530751] Chung AC, Xu X, Niederreither KA, Cooney AJ 2006 Loss of orphan nuclear receptor GCNF function disrupts forebrain development and the establishment of the isthmic organizer. Dev Biol (293):13-24
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE