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EMAGE:4755

Olig1 oligodendrocyte transcription factor 1 ( MGI:1355334)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4755
Figure 4B. Copyright: Reprinted with permission from Cell Press from [doi:10.1016/S0896-6273(00)80897-1] Neuron 25: 317-29, Lu QR; Yuk D; Alberta JA; Zhu Z; Pawlitzky I; Chan J; McMahon AP; Stiles CD; Rowitch DH, "Sonic hedgehog--regulated oligodendrocyte lineage genes encoding bHLH proteins in the mammalian central nervous system."Copyright 2000. [PMID:10719888]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: spinal cord (closed arrow); prosomere 2/3 boundary (open arrow (zli = zona limitans intrathalamica)); telencephalon (tel); diencephalon (di); nasal placode (np); optic vesicle(ov); otic (ot) vesicle.
Expression Pattern Description
Spatial Annotation:
EMAGE:4755Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4755_wholemount_moderate.wlz
4755_wholemount_possible.wlz
4755_wholemount_notDetected.wlz
4755_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4755_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
future spinal cord
detected detected
gradedExpression decreasing caudal-to-rostral.
otocyst
detected detected
diencephalon
detected detected
regionalExpression detected at the prosomere 2/3 boundary (open arrow) separating the telencephalon (tel) from the diencephalon (di).
telencephalon
detected detected
regionalExpression detected at the prosomere 2/3 boundary (open arrow) separating the telencephalon (tel) from the diencephalon (di).
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Olig1 probeA
Entity Detected:Olig1, oligodendrocyte transcription factor 1 ( MGI:1355334)
Sequence:sense strand is shown

>Olig1 probeA
CGGGGCGGGGCGCGACCTCAGCCAATCTTCCCGCGCCCTCGCGTCCTGGATCTAGGGAGAGCTGGCCCCA
GAAAGGAGGACATTTCCAGACTTCTCACCCAGACGACAGACTGTAGGGCGCCTTCTTCCCCGCCCCCACT
CCCTGGCAATTAAAGTGACCCAAGCGGGTGTTCCAAGGAGCGACGCAGTTGCCTGGGATTCTAGATGAAT
CCCACCAGTTTAGAGCCAGAAGTCATTGCCTCCCCACCATAAGCTCACCCAGTAGCGGAGGTGACACCTC
CACCCGGTGGTCTGGTTTTGTACCCGCGAGCATCGCGGAGACCGGGAGGATGGTAGCCGGCGGTGCGGTA
CAATGGGGTTGGGGTTGCCCTGGGTGCAGCGAAAGGCTGTCCCCGAGCTTGGTGCCCTGGCATCCGCCAG
TTCCCTGGCCTAAGAGCTGCTCCCCAACAGTGTCCACTCAGAGGGGCCTCTTTCCTTGTCTGGGGCCTGA
GCGCATAGGCTCCCTTGCTCTCACCAGCCAAGCCCATGCGGATCTAGGAGGTCTAGAAAGCCCAGGCCAC
GAGTACAAACATCAAGGGTGTTGCCGATGGGCCGCTCCGAAAAGCCCAGAGTCCGGTGTCCCGCGAGTGC
GCGTGGTGACAGCGCCTCGGTCGTTGGGTTTCGGAGTGGAGAAGTTCCTCTGGCTGGCCTCACAGTTCTG
TCTTTCAGGCTCGCACACCCAGGTGTTTTGTTGCAATTTACACTCCTTAGTCTCTGCTTCCCCTAATGGT
AGCTTAACCAACATTTGAGCTTGCTTGAAAACAAAATTCCAACCACGTTCCACGCCATCGGTGTTCTGGA
CTTACTAAAATAGATAGCAAGGCATGTCGTAGCGTAGGCTTGTGAAGCTCGGTAATTAACGTTCTCACTA
AAGTAAGCGGGCGAG
nt 1119 - nt 2043 of AF151367.1
Notes:The Olig1 (Olg-1) probe used in this study by Lu et al., 2000 [PMID:10719888] is described as follows: "A 926 bp SmaI-BamHI 3' fragment of Olg-1 3' untranslated region was cloned into pBluescript KSII, creating pBSRGUTR. Antisense digoxigenin riboprobe for Olg-1 was generated by linearization of pBSRGUTR with SmaI and subsequent incubation with T7 RNA polymerase. "
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:Swiss Webster B
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Lu QR; Yuk D; Alberta JA; Zhu Z; Pawlitzky I; Chan J; McMahon AP; Stiles CD; Rowitch DH, 2000 [PMID:10719888] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0896-6273(00)80897-1] [ PMID:10719888] Lu QR, Yuk D, Alberta JA, Zhu Z, Pawlitzky I, Chan J, McMahon AP, Stiles CD, Rowitch DH 2000 Sonic hedgehog--regulated oligodendrocyte lineage genes encoding bHLH proteins in the mammalian central nervous system. Neuron (25):317-29
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE