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EMAGE:4756

Olig2 oligodendrocyte transcription factor 2 ( MGI:1355331)
TS16 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4756
Figure 4C. Copyright: Reprinted with permission from Cell Press from [doi:10.1016/S0896-6273(00)80897-1] Neuron 25: 317-29, Lu QR; Yuk D; Alberta JA; Zhu Z; Pawlitzky I; Chan J; McMahon AP; Stiles CD; Rowitch DH, "Sonic hedgehog--regulated oligodendrocyte lineage genes encoding bHLH proteins in the mammalian central nervous system."Copyright 2000. [PMID:10719888]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: spinal cord (closed arrow); prosomere 2/3 boundary (open arrow); telencephalon (tel); nasal placode (np); otic (ot) vesicle.
Expression Pattern Description
Spatial Annotation:
EMAGE:4756Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4756_wholemount_moderate_3D_1.wlz
4756_wholemount_notDetected_3D_1.wlz
4756_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4756_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
diencephalon
detected detected
regionalExpression detected at the prosomere 2/3 boundary (open arrow) separating the telencephalon (tel) from the diencephalon (di).
telencephalon
detected detected
regionalExpression detected at the prosomere 2/3 boundary (open arrow) separating the telencephalon (tel) from the diencephalon (di).
future spinal cord
detected detected
gradedExpression decreasing caudal-to-rostral.
otocyst
detected detected
eye
detected detected
olfactory pit
detected detected
rhombomere 03
detected detected
Olg-1/2 expression in the 10.5 dpc embryo is detected in the ventral spinal cord with an apparent caudal-to-rostral decrease in signal and a rostral boundary approximately at the second rhombomere of the hindbrain
rhombomere 04
detected detected
Olg-1/2 expression in the 10.5 dpc embryo is detected in the ventral spinal cord with an apparent caudal-to-rostral decrease in signal and a rostral boundary approximately at the second rhombomere of the hindbrain
rhombomere 05
detected detected
Olg-1/2 expression in the 10.5 dpc embryo is detected in the ventral spinal cord with an apparent caudal-to-rostral decrease in signal and a rostral boundary approximately at the second rhombomere of the hindbrain
rhombomere 06
detected detected
Olg-1/2 expression in the 10.5 dpc embryo is detected in the ventral spinal cord with an apparent caudal-to-rostral decrease in signal and a rostral boundary approximately at the second rhombomere of the hindbrain
rhombomere 07
detected detected
Olg-1/2 expression in the 10.5 dpc embryo is detected in the ventral spinal cord with an apparent caudal-to-rostral decrease in signal and a rostral boundary approximately at the second rhombomere of the hindbrain
rhombomere 08
detected detected
Olg-1/2 expression in the 10.5 dpc embryo is detected in the ventral spinal cord with an apparent caudal-to-rostral decrease in signal and a rostral boundary approximately at the second rhombomere of the hindbrain
rhombomere 02
possible possible
Olg-1/2 expression in the 10.5 dpc embryo is detected in the ventral spinal cord with an apparent caudal-to-rostral decrease in signal and a rostral boundary approximately at the second rhombomere of the hindbrain
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Olig2 probeA
Entity Detected:Olig2, oligodendrocyte transcription factor 2 ( MGI:1355331)
Notes:The Olig2 (Olg-2) probe used in this study by Lu et al., 2000 [PMID:10719888] was generated by linearisation of the plasmid pRBRAH16 (ATCC) with EcoRI and subsequent incubation with T3 RNA polymerase".
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Strain:Swiss Webster B
Age:10.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Lu QR; Yuk D; Alberta JA; Zhu Z; Pawlitzky I; Chan J; McMahon AP; Stiles CD; Rowitch DH, 2000 [PMID:10719888] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0896-6273(00)80897-1] [ PMID:10719888] Lu QR, Yuk D, Alberta JA, Zhu Z, Pawlitzky I, Chan J, McMahon AP, Stiles CD, Rowitch DH 2000 Sonic hedgehog--regulated oligodendrocyte lineage genes encoding bHLH proteins in the mammalian central nervous system. Neuron (25):317-29
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE