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EMAGE:4763

Pbx3 pre B-cell leukemia transcription factor 3 ( MGI:97496)
TS16 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4763
Figure 1B. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2005.12.002] Gene Expr Patterns 6: 747-757, Di Giacomo G; Koss M; Capellini TD; Brendolan A; Popperl H; Selleri L, "Spatio-temporal expression of Pbx3 during mouse organogenesis." Copyright 2006. [PMID:16434237]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4763Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4763_wholemount_moderate_3D_1.wlz
4763_wholemount_weak_3D_1.wlz
4763_wholemount_notDetected_3D_1.wlz
4763_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4763_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
regionalExpression is evident in the head.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Pbx3 probeA
Entity Detected:Pbx3, pre B-cell leukemia transcription factor 3 ( MGI:97496)
Notes:The Pbx3 probe template used in this study by Di Giacomo et al., 2006 [PMID:16434237] was "a 2.3 kb Pbx3a clone (clone 2A), spanning 3' UTR sequences, isolated from an embryonic mouse cDNA library (this unpublished clone largely overlaps a clone assigned GenBank Accession No. AF020199)".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:10.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Di Giacomo G; Koss M; Capellini TD; Brendolan A; Popperl H; Selleri L, 2006 [PMID:16434237] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.modgep.2005.12.002] [ PMID:16434237] Di Giacomo G, Koss M, Capellini TD, Brendolan A, P�pperl H, Selleri L 2006 Spatio-temporal expression of Pbx3 during mouse organogenesis. Gene Expr Patterns (6):747-57
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE