Type: | in situ hybridisation probe |
Identifier: | Pcdh8 probeA |
Entity Detected: | Pcdh8, protocadherin 8 ( MGI:1306800) |
Sequence: | sense strand is shown
>Pcdh8 probeA
CAGCGAGGTGATACCGGCTTCCTGTGTCCCGGACCCAGTCGACGAGGCCGCGTCCGCTCCTCCGAGTGCG
GCGGCAGCGGCGGCGGCGGCGAAGGGCGAGGTGGCTGGAGCCCCCGGGGCGGCCAGAGGAGTGATAGAAA
TTTCAGGAGCGTTGTCGTTGACGTCTCGGATGCGCACGATAACCTTGCAGGTGGCTGTCCGGGGCCCTGG
ACCTCGGTCTTGAGCCCGCACGTCCAGCTCGTAGGTGTCCTGGCGCTCGTAGTCCACCTGCCCAGCCAAG
GTGAGGCGGCCAGAGCGCGGGTCGAGCCGGAAGAGGTGGCGGGCTTCGGGAGGGGTGCGGGCGCCGAAGG
TGAACACAACGTCGCCGTTGGGACCTTCGTCGGGATCGGCAGCGTCCAGGTCGAGCAGCAGCGATCCCAC
AGGTGCGTCCTCGGCCAATTCCACCTCTGCCACGGCACCCTGTGGGAAGGCCGGGCTGTGGTCATTGGCA
TCTAGCACACGCACGCTGAGGGCTGCTGTGGCGGAACGCGGCGGTCGCCCGCCATCCTGAGCCACCAGCT
CCAGGCTGTAGGAGGCCTGGCTCTCGCGGTCCAACTCCTGTAGCAGCACCAGGTCCGCACACTGGGCACC
GTCCGCGCGCGTCTGCAGCTCCACGCGGAAGGGGCTGTGAGGCTCGGCTAGGCGTACACTCTGCAGCCCG
TTGGCACCCACGTCCTCGTCCACGGGCACCTCCAGGGGGATGCGTGTGCCTACCGGAGCGCTCTCTGAGA
CCTCCACCGGGATCTGGGCTCGGGGAAAGCGGGGCGCGTGGTCGTTTACATCCCTCACCTCCACCTCCAC
GTGCACCAGGCGGAACTGTTCCTGCGAGAAGCTGACAACATCGAAGGCCAGCACGCACTGTGGGGACGGG
CCACACAGACGCTCACGGTCCAGGCCCGCATCCCCGACGGTCAGCTGCCCGTCGCCCTCGCGTACCCGGA
GCAGAGAGCTGTTGAATTGCTTCATCAGGCGGAAGCTTGTGTCTCCAGACACTTTCATGTGCAGGTCTTC
|
| nt 80169729 - nt 80170778 of NC_000080.5 |
Notes: | The Pcdh8 probe template used in this study by Makarenkova et al., 2005 [PMID:15627506] was "generated by genomic PCR. To detect both Pcdh8 isoforms (as used in this assay), a 1053-bp genomic fragment from the first coding exon was amplified (nucleotides 151-1203 from the initiation codon) using the following PCR primers: forward primer, 5' GAAGACCTGCACATGAAAGTGTTTGGAGAC 3'; reverse primer, 5' CAGCGAGGTGATACCGGCTTCATGTGTC 3'. This fragment was initially cloned into a TOPO vector (InVitrogen, Carlsbad, CA). It was then recloned as a HindIII-EcoRI fragment into pLITMUS 28 (New England Biolabs, Beverly, MA), and used as a template to generate a riboprobe by in vitro transcription using T7 polymerase". |
Chemistry: | RNA |
Strand: | antisense |
Label: | digoxigenin |