Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:4773

Pisrt1 polled intersex syndrome regulated transcript 1 ( MGI:3028611)
TS16 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4773
Figure 3A. [doi:10.1016/j.ymgme.2005.06.013] Loffler KA; Combes AN; Wilhelm D; Beverdam A; Bowles J; Koopman P, "Pisrt1, a gene implicated in XX sex reversal, is expressed in gonads of both sexes during mouse development." Mol Genet Metab (2005) 86: 286-92. [PMID:16137905]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4773Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4773_wholemount_moderate_3D_1.wlz
4773_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4773_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Expression is in "most tissues".
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3698017
Entity Detected:Pisrt1, polled intersex syndrome regulated transcript 1 ( MGI:3028611)
Sequence:sense strand is shown

>MGI:3698017
CAGTCTGACAAAGGAAGGTGATGTTTACAGGCTCCAGGTGGGGAAGCAAGGAGATGGTGGTTTCAGGTGT
AGCTGGCTCGATTTTAGGTGTCTAGATATTCTCTCTGGAATTCAAATGCCCACTTACAGCATGGGGCTCG
GCTAGAGCACTGAGTGTCAGGAGCCCAGCTGCCCAGCCTCAAATCCTGCCCTTGCCTTTAACCTGGGCCA
GTTTGGGATCCTTAAGTTACAAATCTTGCCATCTGTAAAATGGGGCCCTTCCCGGTTTTGAAACCTCCAG
GTCAGGCGTCAAGTGTGGTAAGAAGCTTTTGTGTCTGCTTTTGCCATTGTTCTCACYGTGACCCTGGTTC
CTTCAGCACCACCCCCCCCCGACCCCCGTAAATTACTGAATCTGAATTTGCCCTGCCTCACATTTTCAGG
GAAAGASTGATTTTTATTTTGTTCCTCAATATTAAGGTAACTGCAAACATATTGCTGAAAGAAAATAAAA
GGTATGTCTCCCGCTTTAGTCTGCAAT
nt 1 - nt 517 of AY621661.1
Notes:The Pisrt1 probe template used in this study by Loffler et al., 2005 [PMID:16137905] was generated from "PCR derived fragments of the murine Pisrt1" using the primers "Pisrt1M: 5' CAGTCTGACAAAGGAAGGTGA 3', Pisrt1N: 5' ATTGCAGACTAAAGCGGGAGA 3' ", which produced "a 517 bp Pisrt1 product". Editors Note: The 517nt sequence described was submitted to GenBank as AY621661.1.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:Swiss Quackenbush
Age:10.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Loffler KA; Combes AN; Wilhelm D; Beverdam A; Bowles J; Koopman P, 2005 [PMID:16137905] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.ymgme.2005.06.013] [ PMID:16137905] Loffler KA, Combes AN, Wilhelm D, Beverdam A, Bowles J, Koopman P 2005 Pisrt1, a gene implicated in XX sex reversal, is expressed in gonads of both sexes during mouse development. Mol Genet Metab (86):286-92
Links:MGI:3698030 same experiment
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI