EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:4776

Plagl1 pleiomorphic adenoma gene-like 1 ( MGI:1100874)
TS18 (10.5 dpc)
in situ hybridisation

Data Images


Figure 2A. Copyright: This image is from [doi:10.1002/dvdy.20577] Alam S; Zinyk D; Ma L; Schuurmans C, "Members of the Plag gene family are expressed in complementary and overlapping regions in the developing murine nervous system." Dev Dyn 2005 Nov;234(3): 772-82. [PMID:16193498]	Figure 2G. Copyright: This image is from [doi:10.1002/dvdy.20577] Alam S; Zinyk D; Ma L; Schuurmans C, "Members of the Plag gene family are expressed in complementary and overlapping regions in the developing murine nervous system." Dev Dyn 2005 Nov;234(3): 772-82. [PMID:16193498]	Figure 2J. Copyright: This image is from [doi:10.1002/dvdy.20577] Alam S; Zinyk D; Ma L; Schuurmans C, "Members of the Plag gene family are expressed in complementary and overlapping regions in the developing murine nervous system." Dev Dyn 2005 Nov;234(3): 772-82. [PMID:16193498]

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Note: a, atrium; b, branchial arch; di, diencephalon; fl, forelimb; hb, hindbrain; m, midbrain; nt, neural tube; o, otic vesicle; ol, olfactory pit; oc, optic cup; t, telencephalon; v, ventricle of heart.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

4776_wholemount_moderate.wlz

4776_wholemount_weak.wlz

4776_wholemount_possible.wlz

4776_wholemount_notDetected.wlz

4776_wholemount_strong.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:4776_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

outflow tract

detected

regional

Expression in the walls of the outflow tract

primitive ventricle

detected

heart atrium

detected

bulbus cordis

detected

telencephalon

detected

regional

Higher levels of expression dorsally

diencephalon

detected

regional

Higher levels of expression dorsally

midbrain

detected

regional

Higher levels of expression dorsally

future spinal cord

strong

regional

Strong expression caudally, but also generally , higher level dorsally.

hindbrain

detected

regional

Higher expression detected ventrally.

eye

detected

regional

Expressed in optic cup.

olfactory pit

detected

branchial arch

detected

Expressed in neural crest derived branchial arches, which contribute extensively to the face, nasal cavities, mouth, larynx, pharynx, and neck

trunk somite

strong

regional

Expressed strongly in the rostral compartments of somites.

gut

detected

regional

Zac1 transcripts were detected in the lining of the developing gut and stomach

forelimb bud

detected

Expressed in the centre of the forelimb.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Plagl1 probeA
Entity Detected:	Plagl1, pleiomorphic adenoma gene-like 1 ( MGI:1100874)
Sequence:	sense strand is shown >Plagl1 probeA CATGGCTCCATTCCGCTGTCAAAAATGTGGCAAGTCCTTCGTCACCCTGGAGAAGTTCACCATTCACAAT TATTCCCACTCCAGGGAGCGCCCATTCAAGTGCTCGAAGGCTGAGTGTGGCAAAGCCTTCGTCTCCAAGT ATAAGCTGATGAGACACATGGCCACACACTCGCCACAGAAGATTCACCAGTGTACTCACTGTGAGAAGAC ATTCAACCGGAAGGACCACCTGAAGAACCACCTCCAGACCCACGATCCCAACAAGATCTCCTACGCGTGT GACGATTGCGGCAAGAAGTACCACACCATGCTGGGCTACAAGAGGCACCTGGCCCTGCACTCGGCGAGCA ATGGCGATCTCACCTGTGGGGTGTGCACCCTGGAGCTGGGGAGCACCGAGGTCCTGCTGGACCACCTCAA GTCTCACGCGGAAGAAAAGGCCAACCAGGCACCCAGGGAGAAGAAATACCAGTGCGACCACTGTGATAGA TGCTTCTACACCCGGAAAGATGTGCGTCGCCACCTGGTGGTCCACACAGGATGCAAGGACTTCCTGTGTC AGTTCTGTGCCCAGAGATTTGGGCGCAAAGACCACCTCACTCGTCACACCAAGAAGACCCACTCCCAGGA GCTGATGCAAGAGAATATGCAGGCAGGAGATTACCAGAGCAATTTCCAACTCATTGCGCCTTCAACTTCG TTCCAGATAAAGGTTGATCCCATGCCTCCTTTCCAGCTAGGAGCGGCTCCCGAGAACGGGCTTGATGGTG GCTTGCCACCCGAGGTTCATGGTCTAGTGCTTGCTGCCCCAGAAGAAGCTCCCCAACCCATGCCGCCCTT GGAGCCTTTGGAGCCTTTGGAGCCTTTGGAGCCTTTGGAGCCGATGCAGTCTTTGGAGCCTTTGCAGCCT TTGGAGCCGATGCAGCCTTTGGAGCCAATGCAGCCTTTGGAGCCGATGCAGCCTTTAGAGCCTTTGGAGC CTCTGGAGCCGATGCAGCCTTTGGAGCCGATGCAGCCTTTGGAGCCTATGCAGCCAATGCTGCCAATGCA GCCAATGCAGCCAATGCAGCCAATGCAGCCAATGCTGCCAATGCAGCCAATGCTGCCAATGCAGCCAATG CAGCCAATGCAGCCAATGCTGCCAATGCCAGAGCCGTCTTTCACTCTGCACCCTGGCGTAGTTCCCACCT CTCCTCCCCCAATTATTCTTCAGGAGCATAAGTATAATCCTGTTCCTACCTCATATGCCCCATTTGTAGG CATGCCCGTCAAAGCAGATGGCAAGGCCTTTTGCAACGTGGGTTTCTTTGAGGAATTTCCTCTGCAAGAG CCTCAGGCGCCTCTCAAGTTCAACCCATGTTTTGAGATGCCTATGGAGGGGTTTGGGAAAGTCACCCTGT CCAAAGAGCTGCTGGTAGATGCTGTGAATATAGCCATTCCTGCCTCTCTGGAGATTTCCTCCCTATTGGG GTTTTGGCAGCTCCCCCCTCCTACTCCCCAGAATGGCTTTGTGAATAGCACCATCCCTGTGGGGCCTGGG GAGCCACTGCCCCATAGGATAACCTGTCTGGCGCAGCAGCAGCCACCGCCACTGCCGCCGCCACCACCGC TGCCACTGCCACAGCCACTGCCAGTGCCACAGCCACTACCACAGCCACAGATGCAGCCACAGTTTCAGTT GCAGATCCAGCCCCAGATGCAGCCCCAGATGCAGCTGCAGCCACTGCAGCTGCAGCTACCACAGCTGCTG CCGCAACTGCAACCTCAGCAGCAGCCTGATCCTGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAG AGCCAGAGCCGGAACCGGAACCGGAGCCAGAGCCAGAGCCAGAACCAGAGCCAGAGGAAGAACAGGAAGA GGCAGAAGAAGAGGCAGAGGAAGGAGCAGAGGAAGGAGCAGAACCAGAGGCACAGGCAGAAGAAGAGGAA GAGGAAGAGGAAGCGGAAGAGCCACAGCCAGAAGAAGCCCAAATAGCAGTGAGTGCTGTGAATCTGGGCC AGCCCCCCCTACCCCCAACTCCCCATATTTTCACAGCTGGCTCCAACACTGCTATCCTGCCCCATTTCCA TCACGCATTTAGATAAATTGGTTTTTAAGAGGGTGCTTCTCTTGTGGGAGAT
	nt 555 - nt 2706 of NM_009538.2
Notes:	The Plagl1 (Zac1) probe template used in this study by Alam et al., 2005 [PMID:16193498] was "amplified by PCR using an E13.5 mouse embryonic cDNA library (ResGen) as a template and using the following primers: Zac1 5': AATCTAGACATGGCTCCATTCCGCTGTCA; Zac1 3' primer: AATCTAGAATCTCCCACAAGAGAAGCACC". The "Zac1 cDNA was cloned into the XbaI site of a pCS2-MT expression vector", and "Zac1 antisense probe was generated with XhoI and T7 polymerase and sense probe with Sph1/SP6".
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	CD-1
Age:	10.5 dpc
Theiler Stage:	TS18
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Fixation:	4% paraformaldehyde
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Alam S; Zinyk D; Ma L; Schuurmans C, 2005 [PMID:16193498] . Indexed and spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1002/dvdy.20577] [ PMID:16193498] Alam S, Zinyk D, Ma L, Schuurmans C 2005 Members of the Plag gene family are expressed in complementary and overlapping regions in the developing murine nervous system. Dev Dyn (234):772-82
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source