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EMAGE:4781

Pofut1 protein O-fucosyltransferase 1 ( MGI:2153207)
TS17 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:4781 EMAGE:4781
Figure 1C Antisense. [doi:10.1073/pnas.0831126100] Copyright: This image is from Shi S & Stanley P, Proc Natl Acad Sci U S A 2003 Apr 29;100(9):5234-9. [PMID:12697902] Copyright 2003 National Academy of Sciences, U.S.A. Figure 1C Sense. [doi:10.1073/pnas.0831126100] Copyright: This image is from Shi S & Stanley P, Proc Natl Acad Sci U S A 2003 Apr 29;100(9):5234-9. [PMID:12697902] Copyright 2003 National Academy of Sciences, U.S.A.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Two different specimens are show, hybridised with eith anti-sense or sense Pofut1 probe.
Expression Pattern Description
Spatial Annotation:
EMAGE:4781Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4781_wholemount_moderate.wlz
4781_wholemount_weak.wlz
4781_wholemount_possible.wlz
4781_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4781_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Expression essentially ubiquitous with some variation in expression level.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Pofut1 probeA
Entity Detected:Pofut1, protein O-fucosyltransferase 1 ( MGI:2153207)
Notes:The Pofut1 probe used in this study by Shi & Stanley, 2003 [PMID:12697902] is described as covering the "Pofut1 coding region". Editors note: the exact start and end points of the probe sequence are not given.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:11.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Shi S & Stanley P, 2003 [PMID:12697902] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1073/pnas.0831126100] [ PMID:12697902] Shi S, Stanley P 2003 Protein O-fucosyltransferase 1 is an essential component of Notch signaling pathways. Proc Natl Acad Sci U S A (100):5234-9
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE