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EMAGE:4782

Por P450 (cytochrome) oxidoreductase ( MGI:97744)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4782
Figure 1C. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2006.10.032] Dev Biol 303: 66-81, Ribes V; Otto DM; Dickmann L; Schmidt K; Schuhbaur B; Henderson C; Blomhoff R; Wolf CR; Tickle C; Dolle P, "Rescue of cytochrome P450 oxidoreductase (Por) mouse mutants reveals functions in vasculogenesis, brain and limb patterning linked to retinoic acid homeostasis." Copyright 2007. [PMID:17126317]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4782Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4782_wholemount_moderate_3D_1.wlz
4782_wholemount_weak_3D_1.wlz
4782_wholemount_possible_3D_1.wlz
4782_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4782_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Transcripts widely expressed, apparently at higher levels in limb regions.
limb
strong strong
Transcripts widely expressed, apparently at higher levels in limb regions.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Por probeA
Entity Detected:Por, P450 (cytochrome) oxidoreductase ( MGI:97744)
Sequence:sense strand is shown

>Por probeA
GCCCACTGGAGCACACCCAGGCTGTGGACTATGTTGAGAAGCTCATGACCAAGGGCCGCTACTCGCTGGA
TGTATGGAGCTAGCAGCTGCCGCCCCCCACCCCTCGCTCCCTGTAATCACGTCCTTAACTTCCTTCTGCC
GACCTCCACCTCTGGTGATTCCTGCCCTGCCTGGACACAGGGAGGCCCAGGGACTGACTCCTGGCCTGAG
TGATGCCCTCCTGGCGCCTTAGGCAGAGCATGGTCCATTGTACCAGGCAGCCTAGCCCAGCCCAGGGCAC
ATGGCAAGAGGGACTGGACCCACCTTTGGGTGATGGGTGCCTTAGGTCCCCAGCAGCTGTACAGAAGGGG
CTCTTCTCTCCACAGAGCTGGGGTGCAGCCCCAACATGTGATTTTGAATGAGTGTAAATAATTTTAAATA
ACCTGGCC
Notes:The Por probe used in this study by Ribes et al., 2007 [PMID:17126317] was generated from "I.M.A.G.E. Consortium clone 1179112".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Ribes V; Otto DM; Dickmann L; Schmidt K; Schuhbaur B; Henderson C; Blomhoff R; Wolf CR; Tickle C; Dolle P, 2007 [PMID:17126317] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.ydbio.2006.10.032] [ PMID:17126317] Ribes V, Otto DM, Dickmann L, Schmidt K, Schuhbaur B, Henderson C, Blomhoff R, Wolf CR, Tickle C, Doll� P 2007 Rescue of cytochrome P450 oxidoreductase (Por) mouse mutants reveals functions in vasculogenesis, brain and limb patterning linked to retinoic acid homeostasis. Dev Biol (303):66-81
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE