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EMAGE:4787

Ppt1 palmitoyl-protein thioesterase 1 ( MGI:1298204)
TS19 (11 dpc)
in situ hybridisation

Data Images
EMAGE:4787
Figure 2D. [doi:10.1016/S0165-3806(99)00115-7] Isosomppi J; Heinonen O; Hiltunen JO; Greene ND; Vesa J; Uusitalo A; Mitchison HM; Saarma M; Jalanko A; Peltonen L, "Developmental expression of palmitoyl protein thioesterase in normal mice." Brain Res Dev Brain Res (1999) 118: 1-11. [PMID:10611498]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4787Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4787_wholemount_moderate.wlz
4787_wholemount_weak.wlz
4787_wholemount_possible.wlz
4787_wholemount_notDetected.wlz
4787_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4787_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
regionalSome regionalization of the signal is evident at E11
midbrain
detected detected
regionalsome regionalization of the signal is apparent in the prospective midbrain.
hindbrain
detected detected
regionalsome regionalization of the signal is apparent in the prospective hindbrain.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Ppt1 probeA
Entity Detected:Ppt1, palmitoyl-protein thioesterase 1 ( MGI:1298204)
Notes:The Ppt1 (PPT) probe template used in this study by Isosomppi et al., 1999 [PMID:10611498] "covered nucleotides 123-496" and was "cloned into pBluescript II SK(+/-) vector (Stratagene, CA). Digoxigenin-labeled sense and anti-sense probes were generated using a DIG RNA labeling kit (Boehringer Mannheim)". Editors note: the authors do not refer these co-ordinates to any particular sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:11 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Isosomppi J; Heinonen O; Hiltunen JO; Greene ND; Vesa J; Uusitalo A; Mitchison HM; Saarma M; Jalanko A; Peltonen L, 1999 [PMID:10611498] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0165-3806(99)00115-7] [ PMID:10611498] Isosomppi J, Heinonen O, Hiltunen JO, Greene ND, Vesa J, Uusitalo A, Mitchison HM, Saarma M, Jalanko A, Peltonen L 1999 Developmental expression of palmitoyl protein thioesterase in normal mice. Brain Res Dev Brain Res (118):1-11
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE