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EMAGE:4793

Ptbp1 polypyrimidine tract binding protein 1 ( MGI:97791)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:4793
Figure 2. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(00)00566-9] Mech Dev 2001, 101: 217-20, Lillevali K; Kulla A; Ord T, "Comparative expression analysis of the genes encoding polypyrimidine tract binding protein (PTB) and its neural homologue (brPTB) in prenatal and postnatal mouse brain." Copyright 2001. [PMID:11231079]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: forebrain (FB), midbrain (MB), hindbrain (HB).
Expression Pattern Description
Spatial Annotation:
EMAGE:4793Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4793_wholemount_moderate_3D_1.wlz
4793_wholemount_weak_3D_1.wlz
4793_wholemount_possible_3D_1.wlz
4793_wholemount_notDetected_3D_1.wlz
4793_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4793_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
forebrain
detected detected
Expression is slightly stronger than in the midbrain or hindbrain
hindbrain
detected detected
Expression is slightly weaker than in forebrain.
midbrain
detected detected
Expression is slightly weaker than in forebrain.
embryo
detected detected
Expressed in various parts of the body
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Ptbp1 probeA
Entity Detected:Ptbp1, polypyrimidine tract binding protein 1 ( MGI:97791)
Sequence:sense strand is shown

>Ptbp1 probeA
CGCTCCGTTCCCCGCGGGTCTCTTCCGTGTGCCATGGACGGCATCGTCCCAGACATAGCAGTCGGTACAA
AGCGGGGATCCGACGAGCTCTTCTCCACGTGTGTCAGCAACGGCCCTTCATCATGAGCAGCTCTGCCTCA
GCAGCCAATGGAAACGATAGCAAGAAGTTCAAAGGTGACAACAGGAGCGCAGGAGTCCCTTCCAGAGTCA
TCCATGTCAGAAAGCTGCCCAGCGATGTCACTGAGGGCGAGGTCATCTCCCTAGGGCTGCCCTTTGGAAA
GGTTACCAACCTTCTCATGCTGAAGGGGAAGAACCAGGCCTTCATTGAGATGAACACAGAGGAGGCTGCC
AACACTATGGTTAACTACTATACATCGGTGGCGCCAGTGCTTCGTGGACAGCCCATCTACATCCAGTTCT
CCAACCACAAAGAGCTCAAGACCGACAGCTCGCCCAACCAGGCACGTGCTCAGGCAGCCCTGCAGGCTGT
AAACTCCGTCCAGTCTGTGAACCTGGCCTTGGCAGCCGTCGCTCGTGCCGTGGATTCAGGAATGGCAATG
GCAGGGCAAACGCAGTGCTCAGGATCAATGTGGAAAACTTTTCTATCAGTGAACTGGACGTGTTGACAGA
TCTTTCCTAGTTTGG
Notes:The Ptbp1 (PTB) probe used in this study by Lillevali et al., 2001 [PMID:11231079] was "a 1 kb fragment from coding region of mouse PTB cDNA (originating from I.M.A.G.E. EST clone No. 480448)".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Lillevali K; Kulla A; Ord T, 2001 [PMID:11231079] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(00)00566-9] [ PMID:11231079] Lillev�li K, Kulla A, Ord T 2001 Comparative expression analysis of the genes encoding polypyrimidine tract binding protein (PTB) and its neural homologue (brPTB) in prenatal and postnatal mouse brain. Mech Dev (101):217-20
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE