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EMAGE:4800

Cdca4 cell division cycle associated 4 ( MGI:1919213)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4800
Fig3C. from [doi:10.1016/j.gene.2006.01.027] Gene 374: 153-65, Bennetts JS; Fowles LF; Berkman JL; van Bueren KL; Richman JM; Simpson F; Wicking C, Evolutionary conservation and murine embryonic expression of the gene encoding the SERTA domain-containing protein CDCA4 (HEPP). Copyright 2006 [PMID:16546331]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4800Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4800_wholemount_moderate_3D_1.wlz
4800_wholemount_weak_3D_1.wlz
4800_wholemount_notDetected_3D_1.wlz
4800_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4800_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
1st branchial arch mandibular component
strong strong
1st branchial arch maxillary component
strong strong
2nd branchial arch
strong strong
fronto-nasal process
strong strong
trunk somite
detected detected
hindbrain
detected detected
midbrain
detected detected
future spinal cord
detected detected
dorsal root ganglion
weak weak
forelimb bud
strong strong
regionalExpression was detected in distal mesenchyme of the fore- and hindlimb and was also detected at both the anterior and posterior edges of the proximal limb buds.
hindlimb bud
strong strong
regionalExpression was detected in distal mesenchyme of the fore- and hindlimb and was also detected at both the anterior and posterior edges of the proximal limb buds.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3664102
Entity Detected:Cdca4, cell division cycle associated 4 ( MGI:1919213)
Notes:The Cdca4 probe used in this study by Bennetts et al., 2006 [PMID:16546331] is described as being transcribed from a linearised clone.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Bennetts JS; Fowles LF; Berkman JL; van Bueren KL; Richman JM; Simpson F; Wicking C, 2006 [PMID:16546331] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.gene.2006.01.027] [ PMID:16546331] Bennetts JS, Fowles LF, Berkman JL, van Bueren KL, Richman JM, Simpson F, Wicking C 2006 Evolutionary conservation and murine embryonic expression of the gene encoding the SERTA domain-containing protein CDCA4 (HEPP). Gene (374):153-65
Links:MGI:3664104 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI