Type: | in situ reporter |
Entity Detected: | Cdh1, cadherin 1 ( MGI:88354) |
Notes: | Cdh1 (E-cadherin) expression was detected in this study by Stemmler et al., 2005 [PMID:15673570] using a gene targeted strategy. A targeting vector was designed to insert a Betageo cassette into the ATG codon a loxP site at the 5' end of exon 2. A second targeting vector inserted a PGK-hygromycin cassette flanked by FRT sites and a single loxP site at the 3' end of intron 2, thereby flanking intron 2 with loxP sites (the generated allele is called Ecad-In2floxFRT (MGI:3574476)). Mice carrying the Ecad-In2floxFRT allele were then crossed with Flpe expressing mice to delete the hygromycin cassette. Exon 2 remained flanked by loxP sites. The resultant allele is called Ecad-In2flox (MGI:3574479).
For a full description of the strategy, see figure 1 in paper. The authors also state that the Ecad-In2flox reporter lines reflects the endogenous E-cadherin expression pattern. |
Labelled with: | LacZ |