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EMAGE:4805

Cdh11 cadherin 11 ( MGI:99217)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4805
Fig5C. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1995.1149] Dev Biol 169(1): 347-58, Kimura Y; Matsunami H; Inoue T; Shimamura K; Uchida N; Ueno T; Miyazaki T; Takeichi M, Cadherin-11 expressed in association with mesenchymal morphogenesis in the head, somite, and limb bud of early mouse embryos. Copyright 1995 [PMID:7750650]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: Arrowhead indicates presomitic mesoderm; mb - mandibular arch; mx - maxillary arch; fl - forelimb.
Expression Pattern Description
Spatial Annotation:
EMAGE:4805Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4805_wholemount_moderate_3D_1.wlz
4805_wholemount_weak_3D_1.wlz
4805_wholemount_notDetected_3D_1.wlz
4805_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4805_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
1st branchial arch mandibular component mesenchyme
strong strong
1st branchial arch maxillary component mesenchyme
strong strong
trunk somite
detected detected
gradedThe onset of cad-11 expression in the somites was strictly correlated with the formation of new somites. In each cycle of new somite formation, the first expression of cad-11 occurred at the caudal region of a cluster of newly condensing mesenchymal cells. As these cells formed closed epithelial vesicles, the entire somite structures became stainable for cad-11. However, at later stages, the dermomyotome gradually lost cad-11 expression, leaving only the sclerotome and core cells positive.
notochord
not detected not detected
homogeneous
limb
detected detected
regionalAs limb buds formed, cad-11 appeared in the distal region of the limb mesenchyme.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Cdh11 probeA
Entity Detected:Cdh11, cadherin 11 ( MGI:99217)
Sequence:sense strand is shown

>Cdh11 probeA
CGGGGGAGGAAGTAGGAAGGGTGAAGGCTAAAGACCCAGACATTGGAGAAAATGGCTTAGTCACATACAA
TATCGTTGATGGAGACGGCATAGAACTGTTTGAAATTACAACAGACTATGAAACACAGGATGGTGTGGTG
AAGCTGAAAAAGCCTGTAGATTTTGAAACCAAAAGAGCTTATAGCTTGAAGATAGAGGCCGCCAATGTTC
ACATTGATCCGAAGTTCATCAGCAATGGACCTTTCAAGGACACTGTGACCGTCAAGATTTCAGTAGAAGA
TGCCGATGAGCCTCCCATGTTCTTGGCCCCAAGTTATATCCATGAAGTTCAAGAAAATGCAGCTGCTGGC
ACTGTGGTTGGGAGAGTACATGCCAAAGACCCAGATGCTGCCAACAGCCCAATAAGGTATTCAATTGATC
GTCATACTGACCTCGACAGGTTTTTCACGATTAATCCAGAAGATGGTTTTATTAAAACTACGAAACCTCT
AGATAGGGAAGAAACTGCCTGGCTCAACATCTCTGTCTTCGCAGCAGAAATTCACAACAGACATCAGGAA
ACCAAAGTCCCAGTGGCCATCAGGGTCCTGGATGTCAATGACAATGCTCCTAAGTTTGCTGCCCCTTATG
AAGGTTTTATCTGTGAGAGCGATCACCCCAAGGCACTCTCCAACCAGCCAATAGTTACAGTTAGTGCAGA
TGACCAGGACGACACAGCCAATGGACCAAGATTTATCTTCAGCCTACCCCCTGAAATCATGCACAACCCA
AACTTCACAGTAAGAGACAACAGAGATAACACTGCA
nt 1299 - nt 2104 of D31963.1
Notes:The Cdh11 (Cad-11) probe used in this study by Kimura et al., 1995 [PMID:7750650] is described as follows: "Digoxigenin (DIG)-UTP-labeled RNA probes for hybridization were prepared by use of the plasmids pSP72-11 and pSP73-11. The pSP72-11 was constructed by inserting the 0.8-kb PstI-SmaI fragment of the mouse cad-11 cDNA (Fig. 1) into a pSP72 vector (Promega). The pSP73-11 was constructed using the pSP73 vector (Promega). EcoRI-linearized pSP72-11 was transcribed with T7 RNA polymerase for making a sense probe, and BamHI-linearized pSP73-11 was transcribed with the polymerase for making an antisense probe."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:involves: ICR * C57BL6
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Kimura Y; Matsunami H; Inoue T; Shimamura K; Uchida N; Ueno T; Miyazaki T; Takeichi M, 1995 [PMID:7750650] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.1995.1149] [ PMID:7750650] Kimura Y, Matsunami H, Inoue T, Shimamura K, Uchida N, Ueno T, Miyazaki T, Takeichi M 1995 Cadherin-11 expressed in association with mesenchymal morphogenesis in the head, somite, and limb bud of early mouse embryos. Dev Biol (169):347-58
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE