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EMAGE:4809

Cdh2 cadherin 2 ( MGI:88355)
TS14 (9 dpc)
in situ hybridisation

Data Images
EMAGE:4809
Fig5B. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1995.1149] Dev Biol 169(1): 347-58, Kimura Y; Matsunami H; Inoue T; Shimamura K; Uchida N; Ueno T; Miyazaki T; Takeichi M, Cadherin-11 expressed in association with mesenchymal morphogenesis in the head, somite, and limb bud of early mouse embryos. Copyright 1995 [PMID:7750650]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: mb - mandibular arch, arrowhead - presomitic mesoderm.
Expression Pattern Description
Spatial Annotation:
EMAGE:4809Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4809_wholemount_moderate_3D_1.wlz
4809_wholemount_weak_3D_1.wlz
4809_wholemount_notDetected_3D_1.wlz
4809_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4809_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
branchial arch
not detected not detected
homogeneous
trunk somite
detected detected
Upon the segregation of dermomyotome and sclerotome, N-cadherin expression became confined to the former.
unsegmented mesenchyme
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Cdh2 probeA
Entity Detected:Cdh2, cadherin 2 ( MGI:88355)
Sequence:sense strand is shown

>Cdh2 probeA
TCTACAAAGGCAGAAGAGAGACTGGGTCATCCCGCCAATCAACTTGCCAGAAAACTCCAGAGGACCCTTT
CCTCAAGAGCTTGTCAGAATCAGGTCTGATAGAGATAAAAACCTTTCCCTGAGATACAGCGTCACTGGGC
CAGGAGCTGACCAGCCTCCAACGGGCATCTTCATTATCAACCCCATCTCAGGACAGCTGTCAGTCACAAA
GCCTCTGGATCGAGAGCTGATAGCCCGGTTTCACTTGAGAGCACATGCAGTGGACATCAATGGCAATCAA
GTGGAGAACCCCATTGACATTGTCATCAATGTTATTGACATGAATGATAACAGACCTGAGTTTCTGCACC
AGGTTTGGAATGGGTCTGTTCCAGAGGGATCAAAGCCTGGGACGTATGTGATGACGGTCACTGCCATTGA
TGCGGATGATCCAAATGCCCTGAATGGAATGCTGCGGTACAGGATCCTGTCCCAGGCGCCCAGCACACCT
TCACCCAACATGTTTACAATCAACAATGAGACTGGGGACATCATCACTGTGGCAGCTGGTCTGGATCGAG
AGAAAGTGCAACAGTATACGTTAATAATTCAAGCCACAGACATGGAAGGCAATCCCACTTATGGCCTTTC
AAACACAGCCACAGCCGTCATCACGGTGACAGATGTCAATGACAATCCTCCAGAGTTTACTGCCATGACT
TTCTACGGAGAAGTCCCTGAGAACAGGGTGGACGTCATTGTAGCCAACCTAACTGTCACGGACAAAGATC
AGCCCCACACGCCGGCCTGGAATGCGGCATACAGAATCAGTGGTGGAGACCCTACAGGAAGGTTTGCCAT
CCTGACAGACCCCAACAGCAATGATGGGCTAGTCACAGTGGTAAAACCAATTGACTTTGAAACGAATAGG
ATGTTTGTCCTTACTGTTGCTGCAGAAAACCAAGTGCCATTAGCTAAAGGCATTCAGCACCCACCTCAG
nt 791 - nt 1769 of M31131.1
Notes:The Cdh2 (N-cadherin) probe template used in this study by Kimura et al., 1995 [PMID:7750650] is indicated as "pbMN-3sk+", described by Shimamura et al., 1992 [PMID:1644219] . Editors Note: Shimamura describe the plasmid "pbMN-3", which "was generated by inserting 983 bp of the SacI-SalI fragment of mouse N-cadherin cDNA (Miyatani et al., 1989 [PMID:2762814] ) into the Bluescript vector". "EcoRI-linealized pbMN-3 sk+ plasmid was transcribed using T7 polymerase to generate anti-sense probe".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:involves: ICR * C57BL/6
Age:9 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Kimura Y; Matsunami H; Inoue T; Shimamura K; Uchida N; Ueno T; Miyazaki T; Takeichi M, 1995 [PMID:7750650] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.1995.1149] [ PMID:7750650] Kimura Y, Matsunami H, Inoue T, Shimamura K, Uchida N, Ueno T, Miyazaki T, Takeichi M 1995 Cadherin-11 expressed in association with mesenchymal morphogenesis in the head, somite, and limb bud of early mouse embryos. Dev Biol (169):347-58
 [ doi:10.1016/0012-1606(92)90132-Z] [ PMID:1644219] Shimamura K, Takahashi T, Takeichi M 1992 E-cadherin expression in a particular subset of sensory neurons. Dev Biol (152):242-54
 [ doi:10.1126/science.2762814] [ PMID:2762814] Miyatani S, Shimamura K, Hatta M, Nagafuchi A, Nose A, Matsunaga M, Hatta K, Takeichi M 1989 Neural cadherin: role in selective cell-cell adhesion. Science (245):631-5
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE