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EMAGE:481

Serpine2 serine (or cysteine) peptidase inhibitor, clade E, member 2 ( MGI:101780)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:481
Fig 1A, Kury et al, 1997 [PMID:9102311] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: mm, met-/mesencephalic junction; m, mesencephalon; d, diencephalon; ov, otic vesicle.
Expression Pattern Description
Spatial Annotation:
EMAGE:481Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
481_wholemount_strong_3D_1.wlz
481_wholemount_moderate_3D_1.wlz
481_wholemount_weak_3D_1.wlz
481_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:481_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future midbrain
detected detected
regionalPN-1 transcripts were detected in the ventral neural tube at the met-/mesencephalon junction. PN-1 mRNA was also observed in the ventral part of the mes- and diencephalon
diencephalon
detected detected
regionalPN-1 mRNA was also observed in the ventral part of the mes- and diencephalon
tail
detected detected
regionalPN-1 mRNA was also observed in the ventral ectodermal ridge of the tail
telencephalic vesicle
not detected not detected
Staining in the telencephalic vesicles was considered as an artefact since it could also be observed in control embryos either hybridized with a sense mouse PN-1 riboprobe or without a riboprobe
branchial arch
detected detected
regionalPN-1 mRNA was also in the ectoderm of branchial arches.
otocyst
detected detected
regionalPN-1 mRNA was observed at the surface of the otic vesicle
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1335333
Entity Detected:Serpine2, serine (or cysteine) peptidase inhibitor, clade E, member 2 ( MGI:101780)
Sequence:sense strand is shown

>MGI:1335333
GGATCCAGGTCTTCAATCAGATCATCAAGTCACGGCCTCATGAGAACGTTGTTGTCTCCCCACATGGGAT
CGCGTCCATCTTGGGCATGCTGCAGCTCGGGGCTGACGGCAAGACAAAGAAGCAGCTCTCCACGGTGATG
CGATATAATGTAAACGGAGTTGGTAAAGTGCTGAAGAAGATCAACAAGGCTATTGTCTCCAAGAAAAATA
AAGACATTGTGACCGTGGCCAATGCTGTGTTTCTCAGGAATGGCTTTAAAATGGAAGTGCCTTTTGCAGT
AAGGAACAAAGATGTGTTTCAGTGTGAAGTGCAGAATGTGAACTTCCAGGACCCAGCCTCTGCCTCTGAG
TCCATCAATTTTTGGGTCAAAAATGAGACCAGGGGCATGATTGATAATCTGCTTTCCCCAAATCTGATCG
ATGGTGCCCTTACCAGGCTGGTCCTCGTTAATGCAGTGTATTTCAAGGGTTTGTGGAAGTCTCGGTTTCA
ACCAGAGAGCACAAAGAAACGGACATTCGTGGCAGGTGATGGGAAATCCTACCAAGTACCCATGTTGGCT
CAGCTCTCTGTGTTCCGCTCAGGGTCTACCAGGACCCCGAATGGCTTATGGTACAACTTCATTGAGCTGC
CCTACCATGGTGAGAGCATCAGCATGCTGATCGCCCTGCCAACAGAGAGCTCCACCCCACTGTCTGCCAT
CATCCCTCACATCACTACCAAGACCATTGATAGCTGGATGAACACCATGGTACCCAAGAGGATGCAGCTG
GTCCTACCCAAGTTCACAGCTGTGGCACAAACAGATCTGAAGGAGCCACTGAAAGCCCTTGGCATTACTG
AGATGTTTGAGCCATCAAAGGCAAATTTTACAAAAATAACAAGGTCAGAGAGCCTTCATGTCTCTCACAT
CTTGCAAAAAGCAAAAATTGAAGTCAGTGAAGATGGAACCAA
nt 253 - nt 1204 of NM_009255.2
Notes:The Serpine2 (PN-1) probe used in this study by Kury et al, 1997 [PMID:9102311] is defined as a "951 bp fragment (BamHI-HindIII) spanning the central part of the mouse PN-1 gene"
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Kury et al, 1997 [PMID:9102311] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9102311] Kury P, Schaeren-Wiemers N, Monard D 1997 Protease nexin-1 is expressed at the mouse met-/mesencephalic junction and FGF signaling regulates its promoter activity in primary met-/mesencephalic cells. Development (124):1251-62
Links:MGI:1335376 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI