Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:4814

Cdh22 cadherin 22 ( MGI:1341843)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4814
Fig7C. from [doi:10.1093/emboj/cdg169] Trokovic R; Trokovic N; Hernesniemi S; Pirvola U; Vogt Weisenhorn DM; Rossant J; McMahon AP; Wurst W; Partanen J. 2003. EMBO J 22(8): 1811-23. FGFR1 is independently required in both developing mid- and hindbrain for sustained response to isthmic signals. [PMID:12682014] © Nature Publishing Group. Reproduced with permission from EMBO J.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotation: Arrowhead points to the midbrain-hindbrain boundary.
Expression Pattern Description
Spatial Annotation:
EMAGE:4814Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4814_wholemount__possible.wlz
4814_wholemount__moderate.wlz
4814_wholemount__weak.wlz
4814_wholemount__notDetected.wlz
4814_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4814_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
future midbrain
detected detected
regionalAt E9.5, PB-cadherin was expressed as a stripe throughout the isthmic region. Weaker expression was detected throughout the midbrain.
future hindbrain
detected detected
regionalAt E9.5, PB-cadherin was expressed as a stripe throughout the isthmic region.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Cdh22 probeA
Entity Detected:Cdh22, cadherin 22 ( MGI:1341843)
Sequence:sense strand is shown

>Cdh22 probeA
CGGCCGCGCCCCCTCCCAGTCCAGCCCGGCATCCCCGAGGCTGAGGTCATCATGTGACGCAGCACCGCTG
CGGTGCCTCTGGTCCATGCTCAGGGGCTTGGCCAGCGCCATCAAGCATGAGGCCACGGCCAGAAGGAGCG
CTCCGGGCGGGGGCTGCGCTGTCGCCTGTGCTGCTGTTTCTGCTACTGCTACCGCTGCTGGGGCACCTGT
GGGCCGCAAGCACACCTGCCCCGTCCTCTTTGTCACCTGGGGCTCAGGAGGACAACCAGCTGGGTGCAGG
CCGAGTGAAGCGCGGTTGGGTGTGGAACCAGTTCTTCGTGGTGGAGGAGTACACGGGGACAGAACCCCTG
TACGTGGGCAAGATCCACTCGGATTCTGACGAAGGGGACGGGACCATCAAGTACACCATCTCGGGCGAGG
GTGCAGGGACCATCTTCCTGATTGACGAGTTGACAGGTGACATCCATGCCACGGAGCGCCTGGACCGGGA
GCAGAAAACCTTCTACACACTCCGGGCCCAAGCCAGGGATCGAGCCACCAACCGTCTGCTGGAGCCCGAG
TCGGAGTTCATCATCAAAGTACAGGACATCAACGACAGTGAACCACGCTTTCTCCACGGCCCCTACATTG
GCAGTGTGGCCGAGCTGTCACCTACAGGCACGTCCGTGATGCAGGTGATGGCCTCTGATGCAGACGACCC
TACTTACGGCAGCAGTGCCCGGCTGGTGTACAGTGTGCTGGACGGCGAGCACCACTTCACAGTGGACCCC
AAGACAGGTGTGATCCGGACAGCCGTGCCTGACCTCGACCGTGAGAGCCAGGAACGCTACGAGGTGGTTA
TTCAAGCCACAGACATGGCAGGCCAGCTGGGTGGCCTCTCAGGTTCCACTACAGTCACCATCGTGGTTAC
TGACGTCAACGACAAC
nt 378 - nt 1303 of NM_174988.3
Notes:The Cdh22 (PB-cadherin) probe used in this study by Trokovic et al., 2003 [PMID:12682014] is indicated as being generated from clone UI-M-BH1-akr-h-03-0-UI. Editors Note: Pairwise BLAST comparison of the 5' and 3' EST reads (BE864445.1 and AW045454.1) of UI-M-BH1-akr-h-03-0-UI against the Cdh22 cDNA RefSeq NM_174988.3 was used to deduce the start and end co-ordinates of the probe sequence with respect to NM_174988.3.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% formaldehyde
General Information
Authors:Trokovic R; Trokovic N; Hernesniemi S; Pirvola U; Vogt Weisenhorn DM; Rossant J; McMahon AP; Wurst W; Partanen J, 2003 [PMID:12682014] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1093/emboj/cdg169] [ PMID:12682014] Trokovic R, Trokovic N, Hernesniemi S, Pirvola U, Vogt Weisenhorn DM, Rossant J, McMahon AP, Wurst W, Partanen J 2003 FGFR1 is independently required in both developing mid- and hindbrain for sustained response to isthmic signals. EMBO J (22):1811-23
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE