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EMAGE:4825

Cdkl5 cyclin-dependent kinase-like 5 ( MGI:1278336)
TS15 (9.5 dpc)
immunohistochemistry

Data Images
EMAGE:4825
Fig3A. from [doi:10.1093/hmg/ddi391] Hum Mol Genet 14(24): 3775-86, Lin C; Franco B; Rosner MR, CDKL5/Stk9 kinase inactivation is associated with neuronal developmental disorders. Copyright 2005 [PMID:16330482]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4825Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4825_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4825_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
homogeneousThere were no distinctive areas of intense staining, indicating that CDKL5 is ubiquitous throughout the embryo. Incubation with a blocking peptide corresponding to the antibody epitope suppressed immunoreactivity, suggesting that the immunostaining is specific.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:3686881
Entity Detected:Cdkl5, cyclin-dependent kinase-like 5 ( MGI:1278336)
Antigen:sense strand is shown

>MGI:3686881
SQPDEVIFLASHEKWKQ
aa 922 - aa 938 of NP_001019795.1
Notes:The Cdkl5 antibody used in this study by Lin et al., 2005 [PMID:16330482] is described as follows: "Anti-CDKL5 rabbit antiserum (Cocalico Biologicals) was raised against a peptide corresponding to the last 17 amino acids of the C-terminal domain of CDKL5. The CDKL5 peptide was synthesized by the University of Chicago Cancer Research Center Peptide Facility and was conjugated to keyhole limpet hemocyanin protein via EDC coupling (Pierce). The anti-CDKL5 antiserum was purified by affinity chromatography using the CDKL5 peptide coupled to cyanogen bromide activated sepharose."
Antibody Type:polyclonal
Raised In:rabbit
Supplier:Cocalico Biologicals
Catalogue Number:n/a (custom production)
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Labelled with:Cy3
Visualisation method:fluorescence
General Information
Authors:Lin C; Franco B; Rosner MR, 2005 [PMID:16330482] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1093/hmg/ddi391] [ PMID:16330482] Lin C, Franco B, Rosner MR 2005 CDKL5/Stk9 kinase inactivation is associated with neuronal developmental disorders. Hum Mol Genet (14):3775-86
Links:MGI:3686883 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI