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EMAGE:4828

Cdkn1a cyclin-dependent kinase inhibitor 1A (P21) ( MGI:104556)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:4828
Fig4u. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2004.11.024] Dev Biol 278(2): 428-39, Trokovic R; Jukkola T; Saarimaki J; Peltopuro P; Naserke T; Weisenhorn DM; Trokovic N; Wurst W; Partanen J; Fgfr1-dependent boundary cells between developing mid- and hindbrain. Copyright 2004 [PMID:15680361]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: The midbrain-hindbrain boundary is marked by arrowheads.
Expression Pattern Description
Spatial Annotation:
EMAGE:4828Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4828_wholemount_moderate.wlz
4828_wholemount_notDetected.wlz
4828_wholemount_strong.wlz
4828_wholemount_possible.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4828_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
hindbrain
detected detected
regionalp21 expression in the wild-type embryos was observed in the narrow stripe at the (midbrain-hindbrain) boundary.
midbrain
detected detected
regionalp21 expression in the wild-type embryos was observed in the narrow stripe at the (midbrain-hindbrain) boundary.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Cdkn1a probeB
Entity Detected:Cdkn1a, cyclin-dependent kinase inhibitor 1A (P21) ( MGI:104556)
Notes:The Cdkn1a (p21) probe used in this study by Trokovic et al., 2005 [PMID:15680361] was "a gift from Bert Vogelstein". It is of unreported sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase
General Information
Authors:Trokovic R; Jukkola T; Saarimaki J; Peltopuro P; Naserke T; Weisenhorn DM; Trokovic N; Wurst W; Partanen J, 2005 [PMID:15680361] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.ydbio.2004.11.024] [ PMID:15680361] Trokovic R, Jukkola T, Saarimaki J, Peltopuro P, Naserke T, Weisenhorn DM, Trokovic N, Wurst W, Partanen J 2005 Fgfr1-dependent boundary cells between developing mid- and hindbrain. Dev Biol (2005):428-39
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE