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EMAGE:4843

Cops8 COP9 (constitutive photomorphogenic) homolog, subunit 8 (Arabidopsis thaliana) ( MGI:1915363)
TS15 (9.5 dpc)
immunohistochemistry

Data Images
EMAGE:4843
Fig5B. from [doi:10.1016/S0378-1119(03)00836-9] Gene 321: 65-72, Lykke-Andersen K; Wei N, Gene structure and embryonic expression of mouse COP9 signalosome subunit 8 (Csn8). Copyright 2003 [PMID:14636993]

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4843Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4843_wholemount_moderate_3D_1.wlz
4843_wholemount_weak_3D_1.wlz
4843_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4843_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Csn8 protein was widely expressed in most parts of the embryo.
trunk somite
strong strong
branchial arch
strong strong
cardiovascular system
strong strong
Csn8 (Cops8) appeared to be enriched in the cardio-system.
limb
strong strong
future brain
strong strong
regionalCsn8 (Cops8) appeared to be enriched in parts of the brain.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:Cops8 antibodyA
Entity Detected:Cops8, COP9 (constitutive photomorphogenic) homolog, subunit 8 (Arabidopsis thaliana) ( MGI:1915363)
Notes:The anti-COPS8 (CSN8) antibody used in this study by Lykke-Andersen & Wei, 2003 [PMID:14636993] is referred to as "anti-CSN8 antibody (1:100 dilution, Affiniti Research)." Editors note: Affiniti Research products are distributed through BioMol International Inc., who sell only one anti-CSN8 antibody. The product material sheet for this product (Cat No. PW8290) states "The polyclonal antibody to Csn8 was generated by immunisation of rabbits with recombinant protein corresponding to the full length human protein. The antibody has been characterised by onedimensional Western blotting, immunoprecipitation and by immunohistochemistry". There are two isoforms of human COPS8: NP_937832.1 (160aa) and NP_006701.1 (209aa). It is not stipulated which isoform was used as antigen.
Antibody Type:polyclonal
Raised In:rabbit
Supplier:BioMol International Inc.
Catalogue Number:PW8290
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Secondary Antibody:unspecified (from Sigma)
Labelled with:peroxidase
Visualisation method:DAB
General Information
Authors:Lykke-Andersen K; Wei N, 2003 [PMID:14636993] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0378-1119(03)00836-9] [ PMID:14636993] Lykke-Andersen K, Wei N 2003 Gene structure and embryonic expression of mouse COP9 signalosome subunit 8 (Csn8). Gene (321):65-72
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE