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EMAGE:4847

Crkl v-crk sarcoma virus CT10 oncogene homolog (avian)-like ( MGI:104686)
TS15 (9.5 dpc)
immunohistochemistry

Data Images
EMAGE:4847
Fig1(E9.5) of [doi:10.1038/85855] Guris DL; Fantes J; Tara D; Druker BJ; Imamoto A. 2001. Nat Genet 27(3): 293-8. Mice lacking the homologue of the human 22q11.2 gene CRKL phenocopy neurocristopathies of DiGeorge syndrome. [PMID:11242111] © Nature Publishing Group. Reproduced with permission from Nat Genet.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: fl, forelimb bud; fn, front nasal mass; ht, heart; *, maxillary component of the first branchial arch; 1, first branchial arch; 2, second branchial arch; ov, otic vesicle.
Expression Pattern Description
Spatial Annotation:
EMAGE:4847Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4847_wholemount__moderate.wlz
4847_wholemount__possible.wlz
4847_wholemount__notDetected.wlz
4847_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4847_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
neural tube
strong strong
regionalCrkol is highly expressed in the dorsal neural tube.
branchial arch
strong strong
fronto-nasal process
strong strong
Crkol is highly expressed in the front nasal mass.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:Crkl antibodyA
Entity Detected:Crkl, v-crk sarcoma virus CT10 oncogene homolog (avian)-like ( MGI:104686)
Notes:The anti-Crkl (Crkol) antibody used in this study by Guris et al., 2001 [PMID:11242111] is described as follows: "antibodies to CRKL C-terminal peptide (rabbit polyclonal) from Santa Cruz Biotechnology." Editors note: Santa Cruz Biotech sell one rabbit anti-CRKL antibody raised against the C-terminus:- Crk-L (C-20): sc-319.
Antibody Type:polyclonal
Raised In:rabbit
Supplier:Santa Cruz Biotechnology
Catalogue Number:sc-319
Specimen
Organism:mouse
Strain:129S4/SvJaeSor * C57BL/6J
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:methanol/DMSO 4:1
Secondary Antibody:anti-rabbit IgG
Labelled with:horse radish peroxidase
Visualisation method:4-chloro-1-naphthol
General Information
Authors:Guris DL; Fantes J; Tara D; Druker BJ; Imamoto A, 2001 [PMID:11242111] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1038/85855] [ PMID:11242111] Guris DL, Fantes J, Tara D, Druker BJ, Imamoto A 2001 Mice lacking the homologue of the human 22q11.2 gene CRKL phenocopy neurocristopathies of DiGeorge syndrome. Nat Genet (27):293-8
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE