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EMAGE:4848

Crkl v-crk sarcoma virus CT10 oncogene homolog (avian)-like ( MGI:104686)
TS17 (10.5 dpc)
immunohistochemistry

Data Images
EMAGE:4848
Fig1(E10.5-left) of [doi:10.1038/85855] Guris DL; Fantes J; Tara D; Druker BJ; Imamoto A. 2001. Nat Genet 27(3): 293-8. Mice lacking the homologue of the human 22q11.2 gene CRKL phenocopy neurocristopathies of DiGeorge syndrome. [PMID:11242111] © Nature Publishing Group. Reproduced with permission from Nat Genet.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: fl, forelimb bud; hl, hindlimb bud; tb, tail bud; fn, front nasal mass; ht, heart; DRGs, dorsal root ganglia; *, maxillary component of the first branchial arch; 1, first branchial arch; 2, second branchial arch.
Expression Pattern Description
Spatial Annotation:
EMAGE:4848Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4848_wholemount__moderate.wlz
4848_wholemount__weak.wlz
4848_wholemount__notDetected.wlz
4848_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4848_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
neural tube
strong strong
regionalespecially high concentration in the dorsal part of the neural tube and in neural crest derivatives.
embryo
strong strong
regionalespecially high concentration in neural crest derivatives.
fronto-nasal process
strong strong
especially high concentration in the front nasal mass.
branchial arch
strong strong
dorsal root ganglion
strong strong
cranial ganglion
strong strong
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:Crkl antibodyA
Entity Detected:Crkl, v-crk sarcoma virus CT10 oncogene homolog (avian)-like ( MGI:104686)
Notes:The anti-Crkl (Crkol) antibody used in this study by Guris et al., 2001 [PMID:11242111] is described as follows: "antibodies to CRKL C-terminal peptide (rabbit polyclonal) from Santa Cruz Biotechnology." Editors note: Santa Cruz Biotech sell one rabbit anti-CRKL antibody raised against the C-terminus:- Crk-L (C-20): sc-319.
Antibody Type:polyclonal
Raised In:rabbit
Supplier:Santa Cruz Biotechnology
Catalogue Number:sc-319
Specimen
Organism:mouse
Strain:129S4/SvJaeSor * C57BL/6J
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:methanol/DMSO 4:1
Secondary Antibody:anti-rabbit IgG
Labelled with:horse radish peroxidase
Visualisation method:4-chloro-1-naphthol
General Information
Authors:Guris DL; Fantes J; Tara D; Druker BJ; Imamoto A, 2001 [PMID:11242111] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1038/85855] [ PMID:11242111] Guris DL, Fantes J, Tara D, Druker BJ, Imamoto A 2001 Mice lacking the homologue of the human 22q11.2 gene CRKL phenocopy neurocristopathies of DiGeorge syndrome. Nat Genet (27):293-8
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE