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EMAGE:485

Smad3 MAD homolog 3 (Drosophila) ( MGI:1201674)
TS07 (5.5 dpc)
in situ hybridisation

Data Images
EMAGE:485
Fig 6B Tremblay et al, 2000 [PMID:10862745] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: ExE - extraembryonic ectoderm, EmE - embryonic ectoderm
Expression Pattern Description
Spatial Annotation:
EMAGE:485EMAGE:485Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
485_voxel_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:485_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
trophectoderm
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1860522
Entity Detected:Smad3, MAD homolog 3 (Drosophila) ( MGI:1201674)
Sequence:sense strand is shown

>MGI:1860522
GGATCTCGGTGTGGCGTGGCACCAACACTGGAGGTAGAACTGGCGTCTCTACTCTCTGATAGTGGTAAGG
ATTTACACACACTTCATCCTTCTTCATGTTGAAGGCAAACTCACAGAGCTCCATGGCCCGTAATTCATGG
TGGCTGTGCAGGTCGGGCCATCGCCACAGGCTGCAGTAGATAACGTGAGGGAGCCCCTTCCGATGGGACA
CCTGCAGCCGACCATCCAGTGACCTGGGGATGGTAATGCACTTGGTGTTCACGTTCTGCGTGGTGATGGC
CTTCTCCAGCTCGTCCAACTGCCCCGTCTTCTTGAGCTTCTTCACCAAGCTCTTGACCGCCTTCTCGCAC
CACTTCTCCTCCTGCCCGTTCTGCTCGCCCTTCTTCCAACCCAGCAGGCGCTTCACGATCGGGGGGGTGA
AGGGCAGGATGGACGACATGGCTGGCACCGAAGG
Notes:The Madh3 (Smad3) probe used in this study by Tremblay et al, 2000 [PMID:10862745] was described as "a 424 bp Smad3 EST corresponding mostly to 5'UTR (GenBank accession no. AA023641) ".
Chemistry:RNA
Strand:antisense
Label:unspecified radioactivity
Specimen
Organism:mouse
Age:5.5 dpc
Theiler Stage:TS07
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:autoradiography
General Information
Authors:Tremblay et al, 2000 [PMID:10862745] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10862745] Tremblay KD, Hoodless PA, Bikoff EK, Robertson EJ 2000 Formation of the definitive endoderm in mouse is a Smad2-dependent process. Development (127):3079-90
Links:MGI:1860609 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI