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EMAGE:4852

Cryab crystallin, alpha B ( MGI:88516)
TS15 (9 dpc)
in situ hybridisation

Data Images
EMAGE:4852 EMAGE:4852
Fig2A. Copyright: This image is from [doi:10.1002/(SICI)1097-0177(199609)207:1<75::AID-AJA8>3.0.CO;2-T] Haynes JI 2nd; Duncan MK; Piatigorsky J, "Spatial and temporal activity of the alpha B-crystallin/small heat shock protein gene promoter in transgenic mice." Dev Dyn 1996 Sep;207(1):75-88. Reprinted with permission of Wiley-Liss Inc. [PMID:8875078] . Fig2B. Copyright: This image is from [doi:10.1002/(SICI)1097-0177(199609)207:1<75::AID-AJA8>3.0.CO;2-T] Haynes JI 2nd; Duncan MK; Piatigorsky J, "Spatial and temporal activity of the alpha B-crystallin/small heat shock protein gene promoter in transgenic mice." Dev Dyn 1996 Sep;207(1):75-88. Reprinted with permission of Wiley-Liss Inc. [PMID:8875078] .

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Fig2A shows an embryo hybridised with the antisense probe, Fig2B shows an embryo hybridised with the sense probe. Image annotations: h - heart.
Expression Pattern Description
Spatial Annotation:
EMAGE:4852Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4852_wholemount_moderate_3D_1.wlz
4852_wholemount_notDetected_3D_1.wlz
4852_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4852_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
heart
detected detected
Endogenous alpha B-crystallin mRNA was specifically and exclusively detected in the heart by the antisense probe.
branchial arch
not detected not detected
homogeneous
body cavity or lining
not detected not detected
homogeneous
embryo ectoderm
not detected not detected
homogeneous
limb
not detected not detected
homogeneous
mesenchyme
not detected not detected
homogeneous
notochord
not detected not detected
homogeneous
nervous system
not detected not detected
homogeneous
sensory organ system
not detected not detected
homogeneous
visceral organ system
not detected not detected
homogeneous
venous system
not detected not detected
homogeneous
arterial system
not detected not detected
homogeneous
skeleton
not detected not detected
homogeneous
tail
not detected not detected
homogeneous
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Cryab probeA
Entity Detected:Cryab, crystallin, alpha B ( MGI:88516)
Sequence:sense strand is shown

>Cryab probeA
GACATCGCCATCCACCACCCCTGGATCCGGCGCCCCTTCTTCCCCTTCCACTCCCCAAGCCGCCTCTTCG
ACCAGTTCTTCGGAGAGCACCTGTTGGAGTCTGACCTCTTCTCAACAGCCACTTCCCTGAGCCCCTTCTA
CCTTCGGCCACCCTCCTTCCTGCGGGCACCCAGCTGGATTGACACCGGACTCTCAGAGATGCGTTTGGAG
AAGGACAGATTCTCTGTGAATCTGGACGTGAAGCACTTCTCTCCGGAGGAACTCAAAGTCAAGGTTCTGG
GGGACGTGATTGAGGTCCACGGCAAGCACGAAGAACGCCAGGACGAACATGGCTTCATCTCCAGGGAGTT
CCACAGGAAGTACCGGATCCCAGCCGATGTGGATCCTCTCACCATCACTTCATCCCTGTCATCTGATGGA
GTCCTCACTGTGAATGGACCAAGGAAACAGGTGTCTGGCCCTGAGCGCACCATTCCCATCACCCGTGAAG
AGAAGCCTGCTGTCGCCGCAGCCCCTAAGAAG
nt 523 - nt 1044 of NM_009964.2
Notes:The Cryab (alphaB-crystallin) probe used in this study by Haynes et al., 1996 [PMID:8875078] is described as follows: "A plasmid clone containing a partial alphaB-crystallin cDNA was used in PCR reactions to generate probe templates for in situ hybridization as described by Li et ala, 1994 [PMID:8125186] . The primers used in the generation of the antisense probe template were oligonucleotide 1845 (5' -TAA TAC GAC TCA CTA TAG CTT CTT AGG GGC TGC GGC GAC AGC), which contains the T7 phage promoter and mouse alphaB-crystallin nucleotides +570 to +547, and 1846 (5' -GAC ATC GCC ATC CAC CAC CCC TGG), corresponding to alphaB-crystallin nucleotides +49 to +72. Each PCR reaction resulted in a 540 bp product which was gel-purified and used as a template for the synthesis of digoxigenin-substituted riboprobes." Editors note: the resulting product is in fact 521bp long.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:MEMFA
Staining procedure:alkaline phosphatase
General Information
Authors:Haynes JI 2nd; Duncan MK; Piatigorsky J, 1996 [PMID:8875078] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/(SICI)1097-0177(199609)207:1<75::AID-AJA8>3.0.CO;2-T] [ PMID:8875078] Haynes JI, Duncan MK, Piatigorsky J 1996 Spatial and temporal activity of the alpha B-crystallin/small heat shock protein gene promoter in transgenic mice. Dev Dyn (207):75-88
 [ doi:10.1006/dbio.1994.1077] [ PMID:8125186] Li HS, Yang JM, Jacobson RD, Pasko D, Sundin O 1994 Pax-6 is first expressed in a region of ectoderm anterior to the early neural plate: implications for stepwise determination of the lens. Dev Biol (162):181-94
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE