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EMAGE:4855

Ctgf connective tissue growth factor ( MGI:95537)
TS18 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4855
Fig 1C. Copyright: This image is from Ivkovic S; Yoon BS; Popoff SN; Safadi FF; Libuda DE; Stephenson RC; Daluiski A; Lyons KM, Development 2003; 130(12):2779-91 [doi:10.1242/10.1242/dev.00505] and is displayed with the permission of The Company of Biologist Limited who owns the Copyright. [PMID:12736220]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: ba, branchial arch; h, heart; np, nasal process.
Expression Pattern Description
Spatial Annotation:
EMAGE:4855Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4855_wholemount_moderate_3D_1.wlz
4855_wholemount_weak_3D_1.wlz
4855_wholemount_notDetected_3D_1.wlz
4855_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4855_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
branchial arch
strong strong
regionalhigh levels of Ctgf expression persisted in the proximal branchial arches.
fronto-nasal process
strong strong
high levels of Ctgf expression persisted in the dorsal nasal process.
heart
strong strong
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Ctgf probeA
Entity Detected:Ctgf, connective tissue growth factor ( MGI:95537)
Sequence:sense strand is shown

>Ctgf probeA
TCAGCCAGATCCACTCCAGCTCCGACCCCAGGAGACCGACCTCCTCCAGACGGCAGCAGCCCCAGCCCAG
CCGACAACCCCAGACGCCACCGCCTGGAGCGTCCAGACACCAACCTCCGCCCCTGTCCGAATCCAGGCTC
CGGCCGCGCCTCTCGTCGCCTCTGCACCCTGCTGTGCATCCTCCTACCGCGTCCCGATCATGCTCGCCTC
CGTCGCAGGTCCCATCAGCCTCGCCTTGGTGCTCCTCGCTCTCTGCACCCGGCCTGCTATGGGCCAGGAC
TGCAGCGCGCAATGTCAGTGCGCACGCGAAGCAGCGCCGCACTGCCCCGCCGGCGTGACTAGGTGCTGGA
CGGCTGCGGCTGCTGCGATGCTGCGCCAAGCAGCTGGGAGAACTGTGTACGGAGCGTGACCCCTGCGACC
CACACAAGGGCCTCTTCTGCGATTTCGGCTTCCCGCCAAGCGCAAGATCGGAGTGTGCACTGCAAAGATG
GTGCACCTGTGTCTTCGGTGGGTCGGTGTACGCAGCGGTGAGTCTTCTAAGCAG
Notes:The Ctgf probe used in this study by Ivkovic et al., 2003 [PMID:12736220] is described as "generated by subcloning a partial mouse cDNA IMAGE clone (ID 551901) into pBluescript (Stratagene), linearizing with EcoRI, and reverse transcribing with T7 polymerase."
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Ivkovic S; Yoon BS; Popoff SN; Safadi FF; Libuda DE; Stephenson RC; Daluiski A; Lyons KM, 2003 [PMID:12736220] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.00505] [ PMID:12736220] Ivkovic S, Yoon BS, Popoff SN, Safadi FF, Libuda DE, Stephenson RC, Daluiski A, Lyons KM 2003 Connective tissue growth factor coordinates chondrogenesis and angiogenesis during skeletal development. Development (130):2779-91
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE