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EMAGE:4860

Cyp1b1 cytochrome P450, family 1, subfamily b, polypeptide 1 ( MGI:88590)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4860
Fig3A. from Mol Vis 10: 629-36, Stoilov I; Rezaie T; Jansson I; Schenkman JB; Sarfarazi M, Expression of cytochrome P4501b1 (Cyp1b1) during early murine development. Copyright 2004 [PMID:15359218]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4860Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4860_wholemount_moderate_3D_1.wlz
4860_wholemount_notDetected_3D_1.wlz
4860_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4860_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
forelimb bud
detected detected
regionaldemarcated and asymmetric expression domains could be seen in the optic eminence and forelimb bud. In the forelimb bud Cyp1b1 expression is localized posteriorly.
eye
detected detected
regionaldemarcated and asymmetric expression domains could be seen in the optic eminence and forelimb bud
2nd branchial pouch
not detected not detected
homogeneousCyp1b1 expression is no longer seen in the endoderm and the branchial pouches.
3rd branchial pouch
not detected not detected
homogeneousCyp1b1 expression is no longer seen in the endoderm and the branchial pouches.
2nd branchial arch
detected detected
regionalThe expression domains in the branchial arches are consolidated to relatively small caudal areas on the tip of each arch
3rd branchial arch
detected detected
regionalThe expression domains in the branchial arches are consolidated to relatively small caudal areas on the tip of each arch
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Cyp1b1 probeA
Entity Detected:Cyp1b1, cytochrome P450, family 1, subfamily b, polypeptide 1 ( MGI:88590)
Sequence:sense strand is shown

>Cyp1b1 probeA
AGCTGAGCTCGCTGTCTACCCAGCAGACCACTCTTCTGCTACTCTTCTCCGTCCTGGCCGCCGTGCACTT
AGGCCAGTGGCTGCTGCGACAGTGGCAACGGAAACCGTGGTCCTCGCCCCCAGGTCCCTTTCCTTGGCCA
CTGATCGGAAACGCGGCGGCTGTTGGCCAGGCGTCGCACTTGTACTTCGCTCGCCTTGCAAGGCGCTATG
GCGACGTTTTCCAGATCCGTCTGGGCAGCTGTCCCGTGGTGGTGCTGAATGGAGAGAGTGCCATCCACCA
GGCCCTGGTGCAGCAGGGCAGCATCTTCGCGGACCGGCCGCCCTTCGCCTCTTTCCGTGTGGTGTCTGGT
GGCCGCAGTCTGGCGTTCGGTCACTACTCTGAGCACTGGAAGACGCAGCGACGCTCGGCCTATAGCACGA
TGCGTGCTTTCTCCACGCGCCACCCGCGCAGTCGCGGTCTTCTCGAGGGCCACGCGCTGGCAGAGGCTCG
AGAATTGGTGGCAGTGCTGGTGCGGCGCTGTGCGGGCGGCGCCTTCCTCGATCCAACGCAGCCGGTCATT
GTGGCGGTGGCCAACGTCATGAGCGCTGTGTGCTTCGGCTGTCGGTACAACCACGACGATGCGGAGTTCC
TAGAGCTGCTCAGCCACAATGAGGAGTTCGGGCGCACAGTGGGTGCGGGCAGCCTGGTGGATGTGCTGCC
CTGGCTGCAGCTATTTCCCAACCCGGTGCGCACCACCTTCCGCAAGTTCGAGCAGCTCAACCGCAACTTC
AGCAACTTCGTTCTGGACAAGTTCCTGAGGCACCGCGAAAGCCTGGTGCCCGGGGCTGCTCCTCGAGACA
TGACGGAC
nt 391 - nt 1238 of U03283.1
Notes:The Cyp1b1 probe used in this study by Stoilov et al., 2004 [PMID:15359218] is described as follows: "A 848 bp Cyp1b1 cDNA fragment was amplified by polymerase chain reaction (PCR) by using primer set of 5'-AGC TGA GCT CGC TGT CTA CC-3' and 5'-GTC CGT CAT GTC TCG AGG AG-3' (GenBank accession number U03283, nt 391-1238). This fragment was subcloned into transcription vector pCR�II-TOPO� (Invitrogen, Carlsbad, CA) according to the manufacturer's protocol."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:FVB/NCrlBR
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Stoilov I; Rezaie T; Jansson I; Schenkman JB; Sarfarazi M, 2004 [PMID:15359218] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:15359218] Stoilov I, Rezaie T, Jansson I, Schenkman JB, Sarfarazi M 2004 Expression of cytochrome P4501b1 (Cyp1b1) during early murine development. Mol Vis (10):629-36
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE