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EMAGE:4865

Dab2 disabled homolog 2 (Drosophila) ( MGI:109175)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4865
Fig1A. from [doi:10.1016/j.lfs.2004.10.054] Life Sci 76(22): 2559-68, Airik R; Karner M; Karis A; Karner J, Gene expression analysis of Gata3-/- mice by using cDNA microarray technology. Copyright 2005. [PMID:15769480]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4865Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4865_wholemount_moderate_3D_1.wlz
4865_wholemount_possible_3D_1.wlz
4865_wholemount_notDetected_3D_1.wlz
4865_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4865_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
future hindbrain
detected detected
regionalDab2 expression is located in brain stem.
facio-acoustic vii-viii preganglion complex
detected detected
Dab2 expression is located in the facio-acoustic (VII-VIII) neural crest complex.
branchial arch
detected detected
embryo
detected detected
regionalDab2 expression is located in the mid-embryo region around the first limb buds.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Dab2 probeA
Entity Detected:Dab2, disabled homolog 2 (Drosophila) ( MGI:109175)
Sequence:sense strand is shown

>Dab2 probeA
AAAGGACATTCCCAGTGACGCTTTCACTGGCTTAGACCCCCTTGGGGATAAAGAGGTCAAGGAAGTGAAA
GAAATGTTTAAGGACTTCCAGCTGCGGCAGCCACCTCTTGTTCCCTCAAGGAAGGGGGAGACGCCTCCCT
CTGGGACTTCAAGCGCCTTCTCCAGTTACTTCAACAATAAAGTTGGCATTCCTCAGGAGCATGTAGACCA
TGATGATTTTGATGCCAATCAACTGTTGAACAAGATTAATGAACCACCAAAGCCAGCCCCGAGACAAGGT
GTCCTCTTGGGTACCAAGTCTGCTGACAATTCACTCGAGAACCCTTTCTCCAAAGGGTTCAGCTCATCAA
ACCCCTCTGTGGTTTCTCAGCCTGCATCTTCTGATCCCCACAGGAGCCCTTTCGGAAATCCTTTTGCCTA
GCTTCTGAAGTTGTAATGATGACTATCCAGATGAGCAAAAGACTGGCTTTGGTCAAGAATGAAGCAGACA
GCCAGAAACATGTTGACCTCTGTCCTCGCTC
nt 1447 - nt 1967 of NM_001102400.1
Notes:The Dab2 probe used in this study by Airik et al., 2005 [PMID:15769480] is described as follows: "The mouse Dab2 probe was generated by PCR cloning of a 0.5-kb cDNA from mouse embryonic tail cDNA, using the primers Dab2-F 5'-AAAGGACATTCCCAGTGACG-3' and Dab2-R 5'-GAGCGAGGACAGAGGTCAAC-3'." Editors Note: there are four Dab2 RefSeq cDNA transcript variant sequences (NM_023118.5, NM_001008702.2, NM_001037905.3, NM_001102400.1), however the sequence between the two given primers are identical in each of these four variants.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Airik R; Karner M; Karis A; Karner J, 2005 [PMID:15769480] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.lfs.2004.10.054] [ PMID:15769480] Airik R, Karner M, Karis A, Karner J 2005 Gene expression analysis of Gata3-/- mice by using cDNA microarray technology. Life Sci (2005):2559-68
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE