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EMAGE:4866

Ptn pleiotrophin ( MGI:97804)
TS16 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4866 EMAGE:4866 EMAGE:4866
Fig3f Ptn +/+ top. Copyright: This image is from [doi:10.1002/gene.10091] Loebel DA, O'Rourke MP, Steiner KA, Banyer J, Tam PP., "Isolation of differentially expressed genes from wild-type and Twist mutant mouse limb buds." Genesis 2002 33(3):103-13. Reprinted with permission of Wiley-Liss Inc. [PMID:12124942] Fig3f Ptn +/+ bottom. Copyright: This image is from [doi:10.1002/gene.10091] Loebel DA, O'Rourke MP, Steiner KA, Banyer J, Tam PP., "Isolation of differentially expressed genes from wild-type and Twist mutant mouse limb buds." Genesis 2002 33(3):103-13. Reprinted with permission of Wiley-Liss Inc. [PMID:12124942] Fig3f Ptn +/+ middle. Copyright: This image is from [doi:10.1002/gene.10091] Loebel DA, O'Rourke MP, Steiner KA, Banyer J, Tam PP., "Isolation of differentially expressed genes from wild-type and Twist mutant mouse limb buds." Genesis 2002 33(3):103-13. Reprinted with permission of Wiley-Liss Inc. [PMID:12124942]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Fig3f Ptn +/+ bottom shows a dorsal view of the hindlimb. Fig3f Ptn +/+ middle shows a dorsal view of the forelimb. Limb buds are shown with anterior to the left.
Expression Pattern Description
Spatial Annotation:
EMAGE:4866Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4866_wholemount_moderate.wlz
4866_wholemount_weak.wlz
4866_wholemount_possible.wlz
4866_wholemount_notDetected.wlz
4866_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4866_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
forelimb bud mesenchyme
detected detected
regionalExpression is detected in the proximal (limb) mesenchyme and in the mesenchyme immediately subjacent to the apical ectoderm.
branchial arch
detected detected
neural tube
detected detected
regionalDetected caudally.
embryo
detected detected
regionalExpression is detected in the cranio-facial region
hindlimb bud mesenchyme
detected detected
regionalExpression is detected in the proximal (limb) mesenchyme and in the mesenchyme immediately subjacent to the apical ectoderm.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Ptn probeA
Entity Detected:Ptn, pleiotrophin ( MGI:97804)
Notes:The Ptn probe used in this study by Loebel et al, 2002 [PMID:12124942] was transcribed from a clone indentified through a subtractive hybridisation screen between wild-type and Twist -/- mutant forelimb buds (Ptn was enriched in the mutant). The digoxigenin probe was transcribed using Ampliscribe (Epicentre).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6 * 129
Age:10.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Loebel DA; O'Rourke MP; Steiner KA; Banyer J; Tam PP, 2002 [PMID:12124942] . Indexed and spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/gene.10091] [ PMID:12124942] Loebel DA, O'Rourke MP, Steiner KA, Banyer J, Tam PP 2002 Isolation of differentially expressed genes from wild-type and Twist mutant mouse limb buds. Genesis (33):103-13
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE