EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:4867

Ptprb protein tyrosine phosphatase, receptor type, B ( MGI:97809)
TS16 (10.5 dpc)
in situ reporter

Data Images


Fig 4A. [doi:10.1073/pnas.0611510104] Copyright: This image is from Dominguez MG; Hughes VC; Pan L; Simmons M; Daly C; Anderson K; Noguera-Troise I; Murphy AJ; Valenzuela DM; Davis S; Thurston G; Yancopoulos GD; Gale NW, Proc Natl Acad Sci U S A 2007 Feb 27;104(9):3243-8. [PMID:17360632] Copyright 2007 National Academy of Sciences, U.S.A.	Fig 4B. [doi:10.1073/pnas.0611510104] Copyright: This image is from Dominguez MG; Hughes VC; Pan L; Simmons M; Daly C; Anderson K; Noguera-Troise I; Murphy AJ; Valenzuela DM; Davis S; Thurston G; Yancopoulos GD; Gale NW, Proc Natl Acad Sci U S A 2007 Feb 27;104(9):3243-8. [PMID:17360632] Copyright 2007 National Academy of Sciences, U.S.A.	Fig 4C. [doi:10.1073/pnas.0611510104] Copyright: This image is from Dominguez MG; Hughes VC; Pan L; Simmons M; Daly C; Anderson K; Noguera-Troise I; Murphy AJ; Valenzuela DM; Davis S; Thurston G; Yancopoulos GD; Gale NW, Proc Natl Acad Sci U S A 2007 Feb 27;104(9):3243-8. [PMID:17360632] Copyright 2007 National Academy of Sciences, U.S.A.	Fig 4D. [doi:10.1073/pnas.0611510104] Copyright: This image is from Dominguez MG; Hughes VC; Pan L; Simmons M; Daly C; Anderson K; Noguera-Troise I; Murphy AJ; Valenzuela DM; Davis S; Thurston G; Yancopoulos GD; Gale NW, Proc Natl Acad Sci U S A 2007 Feb 27;104(9):3243-8. [PMID:17360632] Copyright 2007 National Academy of Sciences, U.S.A.	Fig 4E. [doi:10.1073/pnas.0611510104] Copyright: This image is from Dominguez MG; Hughes VC; Pan L; Simmons M; Daly C; Anderson K; Noguera-Troise I; Murphy AJ; Valenzuela DM; Davis S; Thurston G; Yancopoulos GD; Gale NW, Proc Natl Acad Sci U S A 2007 Feb 27;104(9):3243-8. [PMID:17360632] Copyright 2007 National Academy of Sciences, U.S.A.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Image annotations: (A) The lines depict the level of the cross sections in D and E. (B) Head vasculature showing reporter expression in arteries (red arrows), veins (blue arrows) and capillaries. (C) High expression in arterial endothelium (red arrows), especially the aortic sac, outflow tract, and the atrioventricular canal, and lower expression in the cardinal vein (blue arrows). (D) Cross section just above the heart, showing expression in the dorsal aortas, third brachial arch artery, aortic sac, outflow tract (red arrows), and anterior cardinal veins (blue arrows). (E) Cross section just above the forelimbs, showing expression in the fusing dorsal aortas (red arrow) and cardinal veins (blue arrows).

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

4867_wholemount_moderate_3D_1.wlz

4867_wholemount_weak_3D_1.wlz

4867_wholemount_notDetected_3D_1.wlz

4867_wholemount_strong_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:4867_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cardiovascular system

detected

regional

Head vasculature shows reporter expression in arteries (red arrows), veins (blue arrows) and capillaries.

arterial system

strong

regional

High expression in arterial endothelium, especially in the aortic sac, outflow tract and the atrio-ventricular canal.

outflow tract

strong

High expression in arterial endothelium, especially in the aortic sac, outflow tract and the atrio-ventricular canal.

atrioventricular canal

strong

High expression in arterial endothelium, especially in the aortic sac, outflow tract and the atrio-ventricular canal.

cardinal vein

moderate

Expression is less than in the arterial endothelium (especially of the aortic sac, outflow tract, and the atrioventricular canal)

dorsal aorta

detected

3rd arch artery

detected

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ reporter
Entity Detected:	Ptprb, protein tyrosine phosphatase, receptor type, B ( MGI:97809)
Notes:	Ptprb expression was assayed in this study by Dominguez et al., 2007 [PMID:17360632] in mice heterozygous for a targeted mutation, whereby an 11.8-kb segment of the gene extending from 27 nucleotides in exon 2 through exon 3 was replaced with a beta-gal reporter gene and a loxP-flanked neomycin selection cassette. Quantitative RT-PCR on 9.5 embryos demonstrated no gene expression in homozygotes. Beta-gal expression was observed in the developing cardiovascular system.
Labelled with:	LacZ

Specimen

Organism:	mouse
Strain:	involves: 129 * C57BL/6
Age:	10.5 dpc
Theiler Stage:	TS16
Mutations:	Ptprb^tm1Gdy/Ptprb⁺
Preparation:	wholemount

Procedures

General Information

Authors:	Dominguez MG; Hughes VC; Pan L; Simmons M; Daly C; Anderson K; Noguera-Troise I; Murphy AJ; Valenzuela DM; Davis S; Thurston G; Yancopoulos GD; Gale NW, 2007. [PMID:17360632] . Indexed and spatially mapped by EMAGE
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1073/pnas.0611510104] [ PMID:17360632] Dominguez MG, Hughes VC, Pan L, Simmons M, Daly C, Anderson K, Noguera-Troise I, Murphy AJ, Valenzuela DM, Davis S, Thurston G, Yancopoulos GD, Gale NW 2007 Vascular endothelial tyrosine phosphatase (VE-PTP)-null mice undergo vasculogenesis but die embryonically because of defects in angiogenesis. Proc Natl Acad Sci U S A (104):3243-8
Links:	MGI:3763454 allele description
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source