Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:487

Mafb v-maf musculoaponeurotic fibrosarcoma oncogene family, protein B (avian) ( MGI:104555)
TS14 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:487
Fig 6B, Manzanares et al, 1999 [PMID:9895323] . Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: dr - dorsal roof; r5/6 - rhombomeres 5 and 6.
Expression Pattern Description
Spatial Annotation:
EMAGE:487Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
487_wholemount_strong_3D_1.wlz
487_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:487_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future midbrain roof plate
strong strong
regionalIn addition to strong expression in the hindbrain and neural crest migrating into the third branchial arch, another domain is also visible in the dorsal roof over the anterior hindbrain and midbrain.
future hindbrain
strong strong
regionalIn addition to strong expression in the hindbrain and neural crest migrating into the third branchial arch, another domain is also visible in the dorsal roof over the anterior hindbrain and midbrain.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1328161
Entity Detected:Mafb, v-maf musculoaponeurotic fibrosarcoma oncogene family, protein B (avian) ( MGI:104555)
Sequence:sense strand is shown

>MGI:1328161
GGGCCGGCCATGCACACGCCTGCAGCCTGCTGGCTCGGTGTCGTCCACCCCGCTCAGCACTCCGTGCAGC
TCCGTGCCTTCTTCTCCCAGCTTCAGTCCGACTGAACCGAAGACCCATCTCGAGGACCTGTACTGGATGG
CGAGCAACTACCAGCAGATGAACCCCGAGGCACTCAACCTGACGCCCGAGGACGCGGTGGAGGCGCTCAT
CGGTTCGCACCCAGTGCCCCAGCCGCTGCAGAGCTTCGACGGCTTCCGTAGTGCGCACCACCATCACCAC
CACCACCACCCTCATCCGCACCACGGGTACCCAGGAGCAGGTGTGACTCACGATGACCTGGGCCAGCACG
CTCACCCGCACCATCACCATCATCACCAAGCGTCGCCCCCGCCGTCCAGCGCTGCCAGTCCCGCGCAACA
GCTACCCACTAGCCACCCGGGGCCGGGACCGCACGCAACAGCCGCGGCGACGGCTGCGGGCGGCAACGGT
AGTGTGGAGGACCGCTTCTCTGATGACCAGCTGGTGTCCATGTCGGTGCGTGAGCTGAACCGCCACCTGC
GGGGCTTCACCAAGGACGAGGTGATCCGCCTGAAGCAGAAGCGGCGGACCCTGAAGAACCGGGGCTACGC
CCAGTCGTGCAGGTATAAACGCGTCCAGCAGAAACATCACCTGGAGAACGAGAAGACGCAGCTCATTCAG
CAGGTGGAGCAGCTTAAGCAGGAGGTGTCCCGGCTGGCCCGCGAGAGAGACGCCTACAAGGTCAAGTGCG
AGAAACTCGCCAACTCCGGCTTCAGGGAGGCGGGCTCCACCAGCGACAGCCCCTCCTCTCCTGAGTTCTT
TCTGTGAGTCCTGGCGGGTCCGGCCCCCGCCCTTGCCCTGGCCCAGACTCCCTATTCTGCGCCCCTAGCC
CTGGACTCCCTGTCCCTGCCATGGCCCCGGCCTTGACCTGTTTGACTTGAGCTAGAGGGAGGAAGGACGC
GCGGGTCGCGGGAGTCAGGCGGGAGCACGGGCGGGCAGAGAACCTTGGCTAAGAAGAGGGCAGCTCAGGG
CGGCGCAGCCTCTTAGACTTGGGCAGAGTTAGAGAGACCCGGGCGGGTGCGAGGTCCGGGAGTAACTTTT
CTCCAAGCTGGAAGGCCGCGAGGCTTATTCCAAGGAGTCGCCAAGGCCGTCTGGACACTTCTGGCTCTGA
GAACTTTGTGAGTGCCAGGGCCGCCGCTTTGCAGCCCAGGACTCAGTTCGTCCCAGGAAGAGCAGAGGAG
AGGAGAGGGACTCTGGAGTCCCCGTGGGCACGAGGCAAGGCTGAGCTAAGGAAGGAAAAACAGACAGATC
TGTCTGTCCAGAGTACGGAGAACACTGGCTCTCAGCCGGAGACGCGGGCCTCAGTTAGGACTTTCGGCGC
GCG
nt 218 - nt 1620 of NM_010658.1
Notes:The MafB (kreisler) probe used in this study by Manzanares et al, 1999 [PMID:9895323] is described as "a full-length kreisler cDNA probe (Cordes & Barsh, 1994 [PMID:8001130] ) ". Cordes & Barsh depict the sequence of this 'full length' clone (cDNA2) in Fig 3B of that paper. The sequence of cDNA2, in relation to the longer NM_010658 clone, is noted above.
Chemistry:RNA
Strand:antisense
Label:undefined
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:undefined
General Information
Authors:Manzanares et al, 1999 [PMID:9895323] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9895323] Manzanares M, Cordes S, Ariza-McNaughton L, Sadl V, Maruthainar K, Barsh G, Krumlauf R 1999 Conserved and distinct roles of kreisler in regulation of the paralogous Hoxa3 and Hoxb3 genes. Development (126):759-69
 [ doi:10.1016/0092-8674(94)90033-7] [ PMID:8001130] Cordes SP, Barsh GS 1994 The mouse segmentation gene kr encodes a novel basic domain-leucine zipper transcription factor. Cell (79):1025-34
Links:MGI:1328165 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI