| Type: | in situ hybridisation probe |
| Identifier: | MGI:3758729 |
| Entity Detected: | Pycard, PYD and CARD domain containing ( MGI:1931465) |
| Sequence: | sense strand is shown
>MGI:3758729
CCATGGGGCGGGCACGAGATGCCATCCTGGACGCTCTTGAAAACTTGTCAGGGGATGAACTCAAAAAGTT
CAAGATGAAGCTGCTGACAGTGCAACTGCGAGAAGGCTATGGGCGCATCCCACGCGGGGCCCTGCTGCAG
ATGGACGCCATAGATCTCACTGACAAACTTGTCAGCTACTATCTGGAGTCGTATGGCTTGGAGCTCACAA
TGACTGTGCTTAGAGACATGGGCTTACAGGAGCTGGCTGAGCAGCTGCAAACGACTAAAGAAGAGTCTGG
AGCTGTGGCAGCTGCAGCCAGTGTCCCTGCTCAGAGTACAGCCAGAACAGGACACTTTGTGGACCAGCAC
AGGCAAGCACTCATTGCCAGGGTCACAGAAGTGGACGGAGTGCTGGATGCTTTGCATGGCAGTGTGCTGA
CTGAAGGACAGTACCAGGCAGTTCGTGCAGAGACCACCAGCCAAGACAAGATGAGGAAGCTCTTCAGCTT
TGTTCCATCCTGGAACCTGACCTGCAAGGACTCCCTCCTCCAGGCCTTGAAGGAAATACATCCCTACTTG
GTGATGGACCTGGAGCAGAGCTGAG
|
| | nt 213 - nt 797 of NM_023258.4 |
| Notes: | The Pycard (ASC) probes used in this study by Masumoto et al, 2001 [PMID:11139337] are described by the authors as follows: "Antisense and sense RNA probes for in situ hybridization were synthesized by T7 RNA polymerase from EcoRI-digested pSPT19-mASC and HindIII-digested pSPT18-anti-mASC, respectively". Construction of pSPT19-mASC is described as such: "The entire cDNA of mASC was cloned by RT-PCR. Total RNA from the thymus of a BALB/c mouse was isolated", "mASC-specific primers were designed based on the sequences of several EST clones homologous to hASC. Aliquots of 2 ug of total RNA were reverse transcribed using an RNA LA PCR Kit Version 1.1 (Takara) and mASC-specific reverse (5'-TCAAAAATTGTGTATACAAAGTC- 3') and forward (5'-AATTCGGATCCAACGGCAGCAGG-3') primers according to the manufacturers instructions, and the amplified cDNAs were sequenced. The entire open reading frame of mASC amplified by PCR with specific primers (5'-CCGCGAATTCCATGGGGCGGGCACGAG-3' and 5'-ACCAGTCGACTCAGCTCTGCTCCAGGTC-3') was inserted into pSPT19". |
| Chemistry: | RNA |
| Strand: | antisense |
| Label: | digoxigenin |
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