Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:4897

Runx2 runt related transcription factor 2 ( MGI:99829)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4897 EMAGE:4897 EMAGE:4897 EMAGE:4897 EMAGE:4897
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.
EMAGE:4897
Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + NBT/BCIP. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured green and the fluorescence channel depicting autofluorescence has been false-coloured red.
Expression Pattern Description
Spatial Annotation:
EMAGE:4897Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4897_wholemount_strong_3D_1.wlz
4897_wholemount_notDetected_3D_1.wlz
4897_wholemount_moderate_3D_1.wlz
4897_wholemount_weak_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4897_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:RUNX2_probeA
Entity Detected:Runx2, runt related transcription factor 2 ( MGI:99829)
Sequence:sense strand is shown

>RUNX2_probeA
AACAGAGAGCGAGGGGGTATAGGGTGCTGAAGGCAAAAGCTTGCAGAACTCTTAGAATGACTCAGGATTC
AAATGAAGGTGGCCCCACCCCAATCTATCCCCCCACACACAAACACTTTGTGCAGGAGGAATGCTGGGAT
TGGCCTGGTGGTATCACTGAATGATCCCTGGCCTGGATTAGGAAGCCACACTTAGGGATTGGCCCTAAGG
TACCAGGAATGATCACAGTACATCCTCCAAGGAGGGCTGGATCTCAAACTCACACACAATGGCAGATACC
AATGGGTACATGGCCTTCCCTGCTCTTGTTGGCCCTGCCTAATGAAAGCCTCCAATGTCAAAGGTTTCCT
GGAGGTCCCCACAGGTTCTGTTGTCTACTGACATCAGCTACCGGTTGCTACTTCAGCTGACCAGTTCCCC
GAGGACAGCGACTTCATTCGACTTCCTGGAAAGCTGCTGGGGGCCCTCCTTGGGTAAGGCACTTTTTGGG
TTCCCCTGAAT
nt 44069441 - nt 44068941 of NC_000083.4
Notes:This probe was transcribed from template produced by a PCR of whole genomic DNA using primers incorporating T3 and T7 promotors. The antisense probe was transcribed using the T7 polymerase. T3- 5' AATTAACCCTCACTAAAGGATTCAGGGGAACCCAAAAAG 3' T7- 5' TAATACGACTCACTATAGGAACAGAGAGCGAGGGGGTAT 3' Product length: 500bp
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Jacqueline Ramsay, David R Fitzpatrick
Principal investigator:David R FitzPatrick, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Submitted by:Jacqueline Ramsay, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Experiment type:screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE