EMAGE mouse gene expression spatial database

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EMAGE:4900

Barx1 BarH-like homeobox 1 ( MGI:103124)
TS15 (9.5 dpc)
in situ hybridisation

Data Images


Raw signal output, as movie.	All sections along the X-axis, as movie.	All sections along the Y-axis, as movie.	All sections along the Z-axis, as movie.	Photograph prior to 3D imaging.

Photograph prior to 3D imaging.	Sample morphology following 3D imaging, as movie

View 3D opt image:
Download 3D images in woolz format
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + BM Purple. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured yellow and the fluorescence channel depicting autofluorescence has been false-coloured blue.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

4900_wholemount_moderate.wlz

4900_wholemount_weak.wlz

4900_wholemount_notDetected.wlz

4900_wholemount_strong.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:4900_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

1st branchial arch mandibular component

strong

homogeneous

1st branchial arch maxillary component

strong

homogeneous

2nd branchial arch

strong

graded

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Barx1_cloneB
Entity Detected:	Barx1, BarH-like homeobox 1 ( MGI:103124)
Sequence:	sense strand is shown >Barx1_cloneB GCGCATCGCACCTGCCGCTGGAGCTGCAACTCCGCGGGAAGCTGGAAGCAGCTGGCTCTGGTGAACCTGG CGCGAAAGCCAAGAAAGGACGCCGGAGTCGCACCGTATTCACTGAGCTGCAGCTGATGGGGCTGGAGAAA CGCTTCGAGAAGCAGAAGTACCTCTCTACCCCTGACAGAATAGATCTAGCTGAGTCCCTGGGCCTGAGCC AGTTACAGGTGAAGACGTGGTATCAAAATCGGAGGATGAAATGGAAGAAAATAGTGCTGCAAGGTGGCGG CCTGGAGTCCCCCACCAAGCCCAAGGGACGGCCCAAGAAGAACTCCATCCCCACGAGCGAGCAGCTCACG GAGCAAGAGCGTGCCAAGGAGACAGAGAAGCCAGCGGAGACGCCGGGCGAGCCCAGCGACCGAAATTGCG AGGACTGAGCGTCGCCGAGGATGCGGCTGGCGCGGTCGGCGGGGACCCAGGAGCTGGCCCTTCCGCGTCC ATGCCGTTTGCTTTCTAATCATTTCATTACTACTTTGAATGCGGACAATTAAGGGCCAGACAAGGAAGGA CACAGGCCCGGAAGCCAATCCCAGGTGTCAGCGAGCTTCTGTCCCCAGTCTGGGAGACTTGTGTCCAGCG TGGCCGAAGCTTCTGGTCTTTCTCGGACCTCCGCAGTGTGCGGCGCTCCACGCTCATTCACGCCCCGCTC CTTGCCCACACTTTTATCCCCGGCCTCCAGCCGGCCTTCTGGGCCCGGACACCGGCAGGCACACACTTAC TTCCGCGCC
	nt 98 - nt 876 of BC119047.1
Notes:	A Barx1 probe template fragment (951nt) in pBluescript II KS+ was kindly donated by Jean-Francois Brunet, Marseille. The antisense probe was transcribed using T7 polymerase after cutting the template with EcoR1. Probe available on request. Probe length: 778nt
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	CD-1
Age:	9.5 dpc
Theiler Stage:	TS15
Mutations:	none (wild-type)
Preparation:	opt

Procedures

Fixation:	4% paraformaldehyde
Embedding:	agarose
Staining procedure:	alkaline phosphatase + BMpurple

General Information

Authors:	Laura Ward, Mike Dixon
Principal investigator:	Mike Dixon, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Submitted by:	Laura Ward, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Experiment type:	screen
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source