EMAGE mouse gene expression spatial database

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EMAGE:4902

Barx1 BarH-like homeobox 1 ( MGI:103124)
TS18 (11 dpc)
in situ hybridisation

Data Images


Raw signal output, as movie.	All sections along the X-axis, as movie.	All sections along the Y-axis, as movie.	All sections along the Z-axis, as movie.	Photograph prior to 3D imaging.

Photograph prior to 3D imaging.	Photograph prior to 3D imaging.	Photograph prior to 3D imaging. Barx1 expression in maxillary and mandibular components of 1st branchial arch, 2nd branchial arch, region anterior to lens vesicle and in stomach.	Sample morphology following 3D imaging, as movie

View 3D opt image:
Download 3D images in woolz format
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + BM Purple. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured yellow and the fluorescence channel depicting autofluorescence has been false-coloured blue.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

4902_wholemount_moderate_3D_1.wlz

4902_wholemount_weak_3D_1.wlz

4902_wholemount_possible_3D_1.wlz

4902_wholemount_notDetected_3D_1.wlz

4902_wholemount_strong_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:4902_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

1st branchial arch mandibular component

strong

regional

1st branchial arch maxillary component

strong

graded

2nd branchial arch

strong

graded

stomach

moderate

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Barx1_cloneB
Entity Detected:	Barx1, BarH-like homeobox 1 ( MGI:103124)
Sequence:	sense strand is shown >Barx1_cloneB GCGCATCGCACCTGCCGCTGGAGCTGCAACTCCGCGGGAAGCTGGAAGCAGCTGGCTCTGGTGAACCTGG CGCGAAAGCCAAGAAAGGACGCCGGAGTCGCACCGTATTCACTGAGCTGCAGCTGATGGGGCTGGAGAAA CGCTTCGAGAAGCAGAAGTACCTCTCTACCCCTGACAGAATAGATCTAGCTGAGTCCCTGGGCCTGAGCC AGTTACAGGTGAAGACGTGGTATCAAAATCGGAGGATGAAATGGAAGAAAATAGTGCTGCAAGGTGGCGG CCTGGAGTCCCCCACCAAGCCCAAGGGACGGCCCAAGAAGAACTCCATCCCCACGAGCGAGCAGCTCACG GAGCAAGAGCGTGCCAAGGAGACAGAGAAGCCAGCGGAGACGCCGGGCGAGCCCAGCGACCGAAATTGCG AGGACTGAGCGTCGCCGAGGATGCGGCTGGCGCGGTCGGCGGGGACCCAGGAGCTGGCCCTTCCGCGTCC ATGCCGTTTGCTTTCTAATCATTTCATTACTACTTTGAATGCGGACAATTAAGGGCCAGACAAGGAAGGA CACAGGCCCGGAAGCCAATCCCAGGTGTCAGCGAGCTTCTGTCCCCAGTCTGGGAGACTTGTGTCCAGCG TGGCCGAAGCTTCTGGTCTTTCTCGGACCTCCGCAGTGTGCGGCGCTCCACGCTCATTCACGCCCCGCTC CTTGCCCACACTTTTATCCCCGGCCTCCAGCCGGCCTTCTGGGCCCGGACACCGGCAGGCACACACTTAC TTCCGCGCC
	nt 98 - nt 876 of BC119047.1
Notes:	A Barx1 probe template fragment (951nt) in pBluescript II KS+ was kindly donated by Jean-Francois Brunet, Marseille. The antisense probe was transcribed using T7 polymerase after cutting the template with EcoR1. Probe available on request. Probe length: 778nt
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	CD-1
Age:	11 dpc
Theiler Stage:	TS18
Mutations:	none (wild-type)
Preparation:	opt

Procedures

Fixation:	4% paraformaldehyde
Embedding:	agarose
Staining procedure:	alkaline phosphatase + BMpurple

General Information

Authors:	Laura Ward, Mike Dixon
Principal investigator:	Mike Dixon, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Submitted by:	Laura Ward, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Experiment type:	screen
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source