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EMAGE:4903

Barx1 BarH-like homeobox 1 ( MGI:103124)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:4903 EMAGE:4903 EMAGE:4903 EMAGE:4903 EMAGE:4903
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.
EMAGE:4903 EMAGE:4903 EMAGE:4903
Photograph prior to 3D imaging. Photograph prior to 3D imaging. Barx1 expression in maxillary and mandibular components of 1st branchial arch, 2nd branchial arch, region anterior to lens vesicle and in stomach. Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + BM Purple. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured yellow and the fluorescence channel depicting autofluorescence has been false-coloured blue.
Expression Pattern Description
Spatial Annotation:
EMAGE:4903Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4903_wholemount_moderate_3D_1.wlz
4903_wholemount_weak_3D_1.wlz
4903_wholemount_notDetected_3D_1.wlz
4903_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4903_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
stomach
moderate moderate
homogeneous
mandibular process
strong strong
regional
maxillary process
strong strong
regional
2nd branchial arch
strong strong
outer ear
moderate moderate
regional
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Barx1_cloneB
Entity Detected:Barx1, BarH-like homeobox 1 ( MGI:103124)
Sequence:sense strand is shown

>Barx1_cloneB
GCGCATCGCACCTGCCGCTGGAGCTGCAACTCCGCGGGAAGCTGGAAGCAGCTGGCTCTGGTGAACCTGG
CGCGAAAGCCAAGAAAGGACGCCGGAGTCGCACCGTATTCACTGAGCTGCAGCTGATGGGGCTGGAGAAA
CGCTTCGAGAAGCAGAAGTACCTCTCTACCCCTGACAGAATAGATCTAGCTGAGTCCCTGGGCCTGAGCC
AGTTACAGGTGAAGACGTGGTATCAAAATCGGAGGATGAAATGGAAGAAAATAGTGCTGCAAGGTGGCGG
CCTGGAGTCCCCCACCAAGCCCAAGGGACGGCCCAAGAAGAACTCCATCCCCACGAGCGAGCAGCTCACG
GAGCAAGAGCGTGCCAAGGAGACAGAGAAGCCAGCGGAGACGCCGGGCGAGCCCAGCGACCGAAATTGCG
AGGACTGAGCGTCGCCGAGGATGCGGCTGGCGCGGTCGGCGGGGACCCAGGAGCTGGCCCTTCCGCGTCC
ATGCCGTTTGCTTTCTAATCATTTCATTACTACTTTGAATGCGGACAATTAAGGGCCAGACAAGGAAGGA
CACAGGCCCGGAAGCCAATCCCAGGTGTCAGCGAGCTTCTGTCCCCAGTCTGGGAGACTTGTGTCCAGCG
TGGCCGAAGCTTCTGGTCTTTCTCGGACCTCCGCAGTGTGCGGCGCTCCACGCTCATTCACGCCCCGCTC
CTTGCCCACACTTTTATCCCCGGCCTCCAGCCGGCCTTCTGGGCCCGGACACCGGCAGGCACACACTTAC
TTCCGCGCC
nt 98 - nt 876 of BC119047.1
Notes:A Barx1 probe template fragment (951nt) in pBluescript II KS+ was kindly donated by Jean-Francois Brunet, Marseille. The antisense probe was transcribed using T7 polymerase after cutting the template with EcoR1. Probe available on request. Probe length: 778nt
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Laura Ward, Bozena Palinkasova, Mike Dixon
Principal investigator:Bozena Palinkasova, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Submitted by:Laura Ward, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Experiment type:screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE