EMAGE mouse gene expression spatial database

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EMAGE:4914

Rcan1 regulator of calcineurin 1 ( MGI:1890564)
TS18 (10.5 dpc)
in situ hybridisation

Data Images


Figure 2B. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(00)00583-9] Mech Dev Mar;101(1-2):289-92, Casas C; Martinez S; Pritchard MA; Fuentes JJ; Nadal M; Guimera J; Arbones M; Florez J; Soriano E; Estivill X; Alcantara S, "Dscr1, a novel endogenous inhibitor of calcineurin signaling, is expressed in the primitive ventricle of the heart and during neurogenesis." Copyright 2001. [PMID:11231093]

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Note: D, diencephalon; FL, forelimb bud; M, mesencephalon; Mn, mandibular branchial arch; Mx, maxillar branchial arch; PV, primitive ventricle; Rh, rhombencephalon; T, telencephalon; TA, truncus arteriosus.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

4914_wholemount_moderate.wlz

4914_wholemount_notDetected.wlz

4914_wholemount_strong.wlz

4914_wholemount_possible.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:4914_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

heart

detected

bulbus cordis

detected

primitive ventricle

detected

Expression is abruptly stopped at the atrio-ventricular border.

bulbus cordis rostral half

detected

neural tube

detected

telencephalon

detected

diencephalon

detected

regional

Expressed in the caudal hypothalamus.

midbrain

detected

regional

Expressed in pretectum.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Rcan1 probeA
Entity Detected:	Rcan1, regulator of calcineurin 1 ( MGI:1890564)
Sequence:	sense strand is shown >Rcan1 probeA TGGTCCACGTGTGTGAGAGTGACCAAGAGAATGAGGAGGAAGAGGAAGAGATGGAGAGAATGAAGAGACC CAAGCCCAAAATCATCCAGACACGGAGACCGGAGTACACACCCATCCACCTCAGCTGAACCGGCGCGCAG ACTGAGACAGCGCTCTGATCCCACGTGTGGGAAGGAATCTTTTACTGTTGCAGGTGGCTGGCCGTGGCTT TGGAGGGAGCGGCCGTGACCACTGTGGCAGAAATTCCAGTTCATGATGCTCAGAAGAGAGTCAAGGCCTC CTCCCCTTGTTCTGATGCTGCCCCTCAGTCCATGTCCACGGCACCTGGGACTGTCTTGGGCCATTCACTG AATGTGTGGCAGTCACACAAGGACACTGGGGACATCCTGAGANAACTGATAGTTCTTGTAATTGCTCATT TCTAGGTTCTGTTTTTGTCAAGGACAGGNTGACTGGTGNCCCAGGGAGAGATTTCTGTTTCCAACCAGAG TTCAGGGTGCAGCCCCCCCACTGGTAGGCAGAGGCACACCCCCAAAGCCACCAAGTGTCCAGTGCAGAAA GGTGGTGGGACAACCCCCGAACCGTGTGGAATTGTCCTTCTCGTTCCTTCCCCATGGTCTGACGTTCATG CATGTGTATTACTGGGTCCCAGGACCAGCCTTTGTTCACGTAGGAAGTTACGCTGTTTTCCATCTTGGGA AGCCAGGAAAGTTCTGGAACACTCTTACCACGTGTCCTCGTTTTTCTCTGCACATGGGGTCGAGGCTGGA GACATCCACACGTCACCCTGCCTGGTGCATTGTGTCACTTGTGCTTCCCTGCAAAAGAAGTTGATCTTGA GTCCCCAAGTAGAACTTAGCCTAACCGTGGGAGAGCAGCAGGGTTGCTGCCAGCCGTCTGTCTGGGCTGG GAAGGTTTGGTGTGGGTGCTTGCGTTCAGCACCGGTCTCCGTTGTTTCCTTCATGTAGTGGTTTGTGTGT GAATTTCATCCCAGACAGTCTTTGGGAACTGTTGGTTGAGACAGTTTTTCACCCCCATTGGGCGTGAAGA TAAACTTGTAAACTTGTAAATACAGCCAAGAGCATATCTTAGTACCTGTCTGTACCTGGGTGGAAGTAGA GGGCAGAGGGAGTTTCAAGCTACCTGTGACTTGTGTGTGGTAGAGGTTACTGTCATGTGTTTAATGGGGT GATCAGAGCATGTGGAGTCCGTCGTAGCCATGTGTTCCTAGAGGGACACAGTGCCTTTCCCCTTTCATCC AGATGACCACGACACTCGGTGAACATTTGAGAATTTCTGCTTTCCCAGTCATCGTGTGTGTGTCTGGTAA ATAGGTATTCTATTTTGGTCTTACCATTGCCATTACAAAGTTTGTCACTCTGAATAACCTGATGCCAGTT AACAGTGCATGCTTTGGGGACTCCGGAGGTGGCTTCCCCTGGGGAGCAAATGGGTTGATGTTCTCCAAAC CCTGTTTCCAGCGCTCGGTGGGAGGGAGCCCACAGGTGGGAGGCCATCCCGTTGCCGCCGGCCCAGTGTT TCTAGTTCTGCACTTTGAACCGGAGAGGCTAACCTCAAAGATATTTTTTTTAACTGCATTCTATAATAAA TCAGCACAATATGCTCTCTGGAAAAAAAAAAA
	nt 484 - nt 2125 of AF260717.1
Notes:	The Rcan1 (Dscr1) probe template used in this study by Casas et al., 2001 [PMID:11231093] is described as follows: A "partial cDNA clone (accession no. AF260717, nt 484-2125) was obtained", "A 1641 bp fragment of the clone was obtained by polymerase chain reaction (PCR) with M13 primers, purified and used as a template to generate sense and antisense riboprobes for ISH using either T3 or T7 RNA polymerase." Editors Note: nt 484-2125 of AF260717.1 equals 1641 nt in length therefore it is assumed that the entire insert of this clone was used as template.
Chemistry:	RNA
Strand:	antisense

Specimen

Organism:	mouse
Age:	10.5 dpc
Theiler Stage:	TS18
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

General Information

Authors:	Casas C; Martinez S; Pritchard MA; Fuentes JJ; Nadal M; Guimera J; Arbones M; Florez J; Soriano E; Estivill X; Alcantara S, 2001 [PMID:11231093] . Indexed and spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/S0925-4773(00)00583-9] [ PMID:11231093] Casas C, Mart�nez S, Pritchard MA, Fuentes JJ, Nadal M, Guimer� J, Arbon�s M, Fl�rez J, Soriano E, Estivill X, Alc�ntara S 2001 Dscr1, a novel endogenous inhibitor of calcineurin signaling, is expressed in the primitive ventricle of the heart and during neurogenesis. Mech Dev (101):289-92
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source