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EMAGE:4921

Rgs4 regulator of G-protein signaling 4 ( MGI:108409)
TS16 (10 dpc)
in situ hybridisation

Data Images
EMAGE:4921
Figure 4A. Copyright: Reprinted with permission from the Society for Neuroscience from J Neurosci 23: 10613-21, Grillet N; Dubreuil V; Dufour HD; Brunet JF, "Dynamic expression of RGS4 in the developing nervous system and regulation by the neural type-specific transcription factor Phox2b." Copyright 2003. [PMID:14627646]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: Cranial ganglia: trigeminal (V); geniculate and acoustic (VII-VIII); glosopharyngeal (IX); vagal (X). Nascent dorsal root ganglia (DRG); locus coeruleus (arrowhead).
Expression Pattern Description
Spatial Annotation:
EMAGE:4921Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4921_wholemount_moderate.wlz
4921_wholemount_possible.wlz
4921_wholemount_notDetected.wlz
4921_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4921_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
dorsal root ganglion
detected detected
trigeminal v ganglion
detected detected
facio-acoustic vii-viii ganglion complex
detected detected
glossopharyngeal ix ganglion
detected detected
vagus x ganglion
detected detected
glossopharyngeal-vagus ix-x ganglion complex
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Rgs4 probeA
Entity Detected:Rgs4, regulator of G-protein signaling 4 ( MGI:108409)
Sequence:sense strand is shown

>Rgs4 probeA
AGTTAAACAAGATGTGCAAAGGACTTGCAGGTCTGCCGGCTTCCTGCCTGAGGAGTGCAAAGGACATGAA
ACATCGGCTGGGGTTCCTGCTGCAGAAGTCAGATTCCTGCGAACACAGTTCTTCACACAGCAAGAAGGAC
AAAGTAGTAACTTGCCAGAGGGTAAGCCAAGAAGAAGTCAAGAAATGGGCTGAATCGTTGGAAAACCTGA
TTCACCATGAATGTGGACTGGCAGCTTTCAAAGCTTTCCTGAAGTCGGAATACAGCGAGGAGAACATTGA
CTTCTGGATCAGCTGTGAGGAGTACAAGAAGATCAAGTCACCTTCTAAACTAAGTCCCAAAGCCAAGAAG
ATCTACAACGAGTTCATCTCAGTGCAAGCAACAAAAGAGGTGAACCTGGACTCTTGCACCAGAGAGGAGA
CAAGCCGGAACATGTTACAGCCCACAATAACCTGTTTTGATGAAGCCCAGAAGAAGATTTTCAACCTGAT
GGAAAAGGATTCCTACCGCCGCTTCCTCAAGTCTCGATTCTATCTTGACCTGACCAATCCTTCCAGCTGC
GGGGCAGAGAAGCAGAAAGGAGCCAAGAGTTCTGCAGACTGCACTTCCCTGGTCTCCCAGTGTGCCTAAT
TCTCACACAGAGGCAGGGAGCCCAAATGCCAAGAC
nt 109 - nt 773 of NM_009062.3
Notes:The Rgs4 probe used in this study by Grillet et al., 2003 [PMID:14627646] was "synthesized from pKS-mRGS4 (resulting from the subcloning of the coding sequence of RGS4 from pCR2.1-mRGS4) (Nomoto et al., 1997 [PMID:9425263] )". Editors Note: The 678bp region of Rgs4 cloned into pCR2.1 by Nomoto et al, was PCR amplified using the following primers: 5'-GAGCTACGTCAACGGTTAAACAAG-3' and 5'-GTCTTGGCATTTCGGTTCTCTGCC-3'
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Grillet N; Dubreuil V; Dufour HD; Brunet JF, 2003 [PMID:14627646] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:14627646] Grillet N, Dubreuil V, Dufour HD, Brunet JF 2003 Dynamic expression of RGS4 in the developing nervous system and regulation by the neural type-specific transcription factor Phox2b. J Neurosci (23):10613-21
 [ doi:10.1006/bbrc.1997.7802] [ PMID:9425263] Nomoto S, Adachi K, Yang LX, Hirata Y, Muraguchi S, Kiuchi K 1997 Distribution of RGS4 mRNA in mouse brain shown by in situ hybridization. Biochem Biophys Res Commun (241):281-7
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE