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EMAGE:4922

Rhox4b reproductive homeobox 4B ( MGI:1930129)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4922 EMAGE:4922
Fig 5A. [doi:10.1002/dvdy.10413] Copyright: This image is from Jackson M, Baird JW, Nichols J, Wilkie R, Ansell JD, Graham G, Forrester LM, "Expression of a novel homeobox gene Ehox in trophoblast stem cells and pharyngeal pouch endoderm." Dev Dyn 2003 May;228(4):740-4. Reprinted with permission of Wiley-Liss Inc. [PMID:15765518] Fig 5B. [doi:10.1002/dvdy.10413] Copyright: This image is from Jackson M, Baird JW, Nichols J, Wilkie R, Ansell JD, Graham G, Forrester LM, "Expression of a novel homeobox gene Ehox in trophoblast stem cells and pharyngeal pouch endoderm." Dev Dyn 2003 May;228(4):740-4. Reprinted with permission of Wiley-Liss Inc. [PMID:15765518]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: o, otic vesicle; p1-4, pharyngeal pouches 1-4.
Expression Pattern Description
Spatial Annotation:
EMAGE:4922Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4922_wholemount_moderate_3D_1.wlz
4922_wholemount_possible_3D_1.wlz
4922_wholemount_notDetected_3D_1.wlz
4922_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4922_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
foregut
detected detected
regionalThe region of expression stretches along the ventral floor of the foregut from the second to the fourth pharyngeal pouches.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Rhox4b probeA
Entity Detected:Rhox4b, reproductive homeobox 4B ( MGI:1930129)
Notes:The Rhox4b (Ehox) probe used in this study by Jackson et al., 2003 [PMID:14648851] was "synthesized by linearization of pBK-CMV containing the full-length 800-bp Ehox cDNA". Editors Note: elsewhere in the manuscript the authors refer to AF265350 as the Ehox cDNA sequence identifier, however this is 893nt in length. It is unclear which region was used as probe.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Jackson M; Baird JW; Nichols J; Wilkie R; Ansell JD; Graham G; Forrester LM, 2003 [PMID:14648851] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/dvdy.10413] [ PMID:14648851] Jackson M, Baird JW, Nichols J, Wilkie R, Ansell JD, Graham G, Forrester LM 2003 Expression of a novel homeobox gene Ehox in trophoblast stem cells and pharyngeal pouch endoderm. Dev Dyn (228):740-4
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE