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EMAGE:4923

Ring1 ring finger protein 1 ( MGI:1101770)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4923
Fig 5A. [doi:10.1093/emboj/16.19.5930] Schoorlemmer J; Marcos-Gutierrez C; Were F; Martinez R; Garcia E; Satijn DP; Otte AP; Vidal M, EMBO J 1997 Oct 1;16(19):5930-42. "Ring1A is a transcriptional repressor that interacts with the Polycomb-M33 protein and is expressed at rhombomere boundaries in the mouse hindbrain." © Nature Publishing Group. Reproduced with permission from EMBO J.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: forebrain (fb), hindbrain (hb), dorsal spinal cord (sc), optic stalk (os).
Expression Pattern Description
Spatial Annotation:
EMAGE:4923Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4923_wholemount__moderate.wlz
4923_wholemount__weak.wlz
4923_wholemount__notDetected.wlz
4923_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4923_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
central nervous system
detected detected
Expression of Ring1 is restricted to the developing CNS, including the forebrain, hindbrain and dorsal spinal cord.
future spinal cord
detected detected
regionalExpression of Ring1 is in dorsal spinal cord.
future forebrain
detected detected
Expression of Ring1 is restricted to the developing CNS, including the forebrain, hindbrain and dorsal spinal cord.
future hindbrain
detected detected
regionalExpression of Ring1 is more intense at the rhombomere boundaries
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Ring1 probeA
Entity Detected:Ring1, ring finger protein 1 ( MGI:1101770)
Sequence:sense strand is shown

>Ring1 probeA
GAATTCATGGATGGTACAGAGATTGCGGTTTCGCCTCGGTCACTGCATTCTGAGCTCATGTGTCCCATCT
GCCTGGACATGCTGAAGAACACAATGACCACCAAGGAGTGCCTGCATAGGTTCTGCTCGGACTGCATCGT
CACCGCCCTGCGGAGCGGGAACAAGGAGTGCCCTACCTGCCGGAAAAAGCTGGTATCCAAGCGGTCCCTA
CGGCCGGACCCCAACTTCGACGCCCTGATCTCCAAAATCTACCCTAGCCGGGAGGAATACGAGGCCCATC
AGGACCGGGTGCTCATCCGCCTCAGCCGCCTGCACAACCAGCAGGCGCTGAGCTCCAGTATCGAGGAAGG
GCTCCGGATGCAGGCCATGCACAGGGCCCAGCGTGTGAGGCGGCCGATGCCTGGATCTGATCAGACCGCC
ACAATGAGTGGGGGGGAAGGAGAACCTGGGGAGGGAGAAGGGGATGGAGAGGATGTAAGCTCTGACTCCG
CCCCAGACTCTGCTCCAGGCCCTGCTCCCAAGCGACCCCGCGGAGCGGGTGCAGGGGCCAGCAGTGTAGG
GACAGGGGGTGGGGCTGCTGGTGGGGCTTGCGGGGGTGCCGGTTCTGAAGACTCTGGTGACCGGGGCGGC
ACCCTGGGAGGGGGAACCCTGGGGCCCCCAAGCCCTCCTGGGGCCCCCAGCCCTCCAGAGCCCGGTGGAG
AGATTGAGCTTGTGTTCCGGCCCCATCCCCTGCTTGTGGAGAAAGGAGAGTACTGCCAGACTCGGTACGT
GAAGACTACTGGGAATGCCACAGTGGATCATCTCTCCAAGTACCTGGCCCTGCGCATTGCCCTGGAGAGG
AGGCAGCAGCAGGAAACCACAGAGCCCGGAGGGCCTGGTGGGGGTGCTTCGGACACAGGCGGACCTGATG
GGGGTGGTGGGGAGAGAGGCGTTGCTGGAGGAGGCGAAGGTCCTGAGGAGCCTGCGCTGCCCAGCCTGGA
AGGTGTCAGCGAAAAGCAGTACACCATCTACATCGCTCCTGGGGGCGGAGCGTTCACGACGCTGAATGGA
TCACTGACCTTGGAGCTTGTAAACGAGAAGTTCTGGAAGGTGTCCAGGCCCTTAGAGCTCTGCTATGCCC
CCACGAAGGACCCAAAATGATGTCACAAGGGGACAGCTGGAACATGGGGACCACAGGGTGTCTCTGTGTC
CCAGCTCAGCCTGCCAGCTTCATCTCCCAAAGCCCCCCAGCCAGACAGCAAGAGGACAAAGGAGGACAAA
TGGCTTTTATACAAAGTATCTATATTGAATTAGTCTATATTGGACAGCACGG
nt 1 - nt 1312 of Y12881.1
Notes:The Ring1 (Ring1A) probe used in this study by Schoorlemmer et al., 1997 [PMID:9312051] was "obtained from pBluescript plasmids (Stratagene) containing full-length Ring1A". Editors note: the authors state that "The murine Ring1A (accession number Y12881) cDNA sequence has been deposited in the DDBJ/EMBL/GenBank database".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Schoorlemmer J; Marcos-Gutierrez C; Were F; Martinez R; Garcia E; Satijn DP; Otte AP; Vidal M, 1997 [PMID:9312051] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1093/emboj/16.19.5930] [ PMID:9312051] Schoorlemmer J, Marcos-Gutierrez C, Were F, Martinez R, Garcia E, Satijn DP, Otte AP, Vidal M 1997 Ring1A is a transcriptional repressor that interacts with the Polycomb-M33 protein and is expressed at rhombomere boundaries in the mouse hindbrain. EMBO J (16):5930-42
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE