EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:4925

Robo1 roundabout homolog 1 (Drosophila) ( MGI:1274781)
TS17 (10.5 dpc)
in situ hybridisation

Data Images


Figure 5K. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1999.9371] Dev Biol 212: 290-306. Yuan W; Zhou L; Chen JH; Wu JY; Rao Y; Ornitz DM, "The mouse SLIT family: secreted ligands for ROBO expressed in patterns that suggest a role in morphogenesis and axon guidance." Copyright 1999 [PMID:10433822]

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Note: II, second branchial arch; limb bud (lb); mandibular (ma) and the maxillary (mx) components of the first branchial arch; nasal pit (np).

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

4925_wholemount_moderate_3D_1.wlz

4925_wholemount_weak_3D_1.wlz

4925_wholemount_possible_3D_1.wlz

4925_wholemount_notDetected_3D_1.wlz

4925_wholemount_strong_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:4925_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

midbrain

weak

nasal process

detected

regional

Expression in the groove between the nasal process and the telencephalon.

telencephalon

detected

regional

1st branchial arch mandibular component

detected

1st branchial arch maxillary component

detected

forebrain

detected

forelimb bud mesenchyme

detected

regional

Expression in the proximal mesenchyme of the limb bud.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Robo1 probeA
Entity Detected:	Robo1, roundabout homolog 1 (Drosophila) ( MGI:1274781)
Sequence:	sense strand is shown >Robo1 probeA ATGAAATGGAAACATCTTCCTCTTCTGGTCATGATATCTCTCCTCACTCTATCAAAAAAGCATCTCCTTC TGGCCCAGCTTATTCCAGACCCTGAGGACCTAGAGAGGGGGAACGACAACGGGACGCCAGCCCCCACCTC TGATAATGATGACAATTCGCTGGGCTACACAGGCTCCCGTCTCCGTCAGGAAGATTTCCCACCGCGCATT GTTGAGCACCCTTCAGACCTGATCGTCTCTAAAGGGGAACCCGCCACCCTCAACTGTAAAGCTGAAGGCC GCCCTACCCCGACTATAGAATGGTACAAGGGCGGCGAAAGAGTGGAAACGGACAAAGACGACCCTCGCTC ACACCGAATGCTGCTGCCAAGTGGATCTTTATTTTTCTTACGCATAGTGCATGGACGGAAGAGTAGGCCT GACGAAGGGGTCTACATCTGTGTAGCAAGGAATTACCTCGGAGAGGCTGTGAGCCACAATGCATCGCTGG AGGTAGCTATACTACGGGATGACTTCAGACAAAACCCTTCAGATGTTATGGTGGCGGTGGGCGAGCCTGC AGTAATGGAATGCCAGCCTCCTCGGGGCCACCCAGAGCCCACCATCTCCTGGAAAAAGGATGGCTCACCC CTGGATGATAAAGACGAGCGAATCACTATCCGAGGCGGGAAGCTCATGATCACGTACACCCGTAAAAGCG ATGCTGGCAAGTATGTTTGTGTTGGTACCAACATGGTAGGGGAGCGGGAGAGCAAAGTCGCGGACGTGAC TGTCTTAGAGAGACCGTCCTTTGTAAAGAGACCCAGTAATTTGGCAGTGACTGTGGATGACAGTGCTGAG TTTAAGTGTGAGGCCCGAGGAGACCCCGTGCCTACGTTCGGGTGGAGGAAAGATGACGGAGAGCTGCCCA AATCCAGATATGAGATCCGAGATGATCATACTTTGAAAATTCGGAAGGTGACAGCTGGCGACATGGGATC ATACACTTGTGTTGCAGAAAATATGGTTGGGAAAGCCGAAGCATCAGCCACTTTGACAGTTCAAGAGCCA CCACATTTTGTCGTGAAGCCTCGAGACCAGGTGGTTGCCTTGGGGCGAACTGTGACTTTTCAGTGTGAAG CAACTGGAAATCCTCAACCAGCTATCTTTTGGCGGAGAGAGGGGAGTCAGAATCTGCTTTTCTCATACCA GCCTCCACAGTCCTCTAGCCGGTTTTCAGTTTCCCAGACGGGGGACCTCACTGTTACGAACGTCCAGAGG TCTGATGTCGGTTACTATATCTGTCAGACTTTGAACGTGGCTGGAAGCATCATCACAAAGGCATATTTGG AAGTTACTGATGTGATTGCGGACCGGCCTCCCCCAGTCATCCGACAAGGTCCTGTGAATCAGACTGTAGC AGTGGATGGTACTTTAACTCTCAGCTGTGTGGCCACAGGCAGTCCCGTGCCCACAATTCTGTGGAGGAAG GATGGAGTCCTTGTTTCAACCCAAGATTCTCGGATCAAACAGCTGGAGAGCGGGGTCCTGCAGATCCGCT ACGCTAAGCTGGGTGACACCGGTCGGTACACCTGCACTGCTTCGACCCCCAGTGGTGAGGCCACGTGGAG CGCTTACATCGAAGTCCAGGAATTTGGAGTTCCAGTTCAACCTCCAAGACCCACCGACCCCAATTTAATC CCCAGTGCGCCTTCAAAGCCTGAAGTCACAGATGTGAGCAAAAACACAGTCACTTTATTGTGGCAGCCTA ACCTGAACTCAGGAGCAACCCCAACGTCTTATATTATAGAAGCCTTCAGCCATGCATCTGGTAGCAGCTG GCAGACCGTGGCAGAAAATGTGAAAACAGAAACATTCGCCATCAAAGGGCTCAAACCTAATGCGATCTAC CTTTTCCTGGTGAGGGCAGCAAATGCCTATGGGATTAGCGATCCAAGCCAAATATCCGATCCAGTGAAAA CACAAGATGTCCCACCAACCACCCAAGGGGTGGACCACAAGCAGGTCCAGAGAGAACTGGGCAATGTTGT CTTGCATCTCCACAACCCTACCATCCTTTCTTCCTCTTCTGTCGAAGTGCACTGGACGGTGGACCAACAA TCTCAATATATTCAAGGTTATAAAATTCTCTACCGACCATCTGGAGCCAGCCATGGCGAATCGGAGTGGT TAGTTTTTGAAGTGAGGACCCCAACCAAAAATAGTGTGGTGATCCCCGATCTGAGAAAAGGCGTCAACTA CGAGATTAAGGCCCGCCCATTTTTTAATGAGTTCCAAGGAGCAGATAGTGAAATCAAATTTGCCAAAACC TTAGAAGAACGACCAAGTGCCCCTCCCCGAAGTGTCACTGTATCCAAGAATGATGGAAATGGGACAGCAA TTCTGGTCACTTGGCAACCGCCTCCTGAAGACACACAGAATGGAATGGTCCAAGAATATAAGGTTTGGTG TCTCGGGAATGAGACTAGGTATCACATAAACAAGACAGTAGATGGCTCTACCTTTTCGGTGGTCATTCCC TTTCTTGTTCCTGGAATCCGCTACAGTGTAGAAGTGGCTGCAAGCACTGGGGCTGGCCCTGGGGTGAAGA GTGAGCCCCAGTTCATCCAGTTGGATTCCCATGGAAACCCCGTGTCTCCCGAGGACCAAGTGAGCCTGGC TCAGCAGATCTCCGACGTGGTGAAGCAGCCAGCCTTCATTGCGGGCATCGGGGCCGCCTGTTGGATCATT CTCATGGTCTTCAGTATCTGGCTTTACCGACACCGCAAGAAGAGAAACGGGCTCAGCAGCACGTACGCGG GGATCCGGAAAGTCCCGTCCTTTACCTTCACACCAACAGTAACTTATCAAAGAGGAGGCGAAGCCGTCAG CAGTGGAGGGAGGCCGGGACTCCTCAACATCAGTGAACCTGCCACACAGCCATGGCTGGCGGACACGTGG CCCAACACAGGCAACAGTCACAATGACTGCTCCATCAACTGCTGCACAGCCAGCAACGGCAACAGCGACA GCAACCTGACCACCTACAGTCGCCCAGCTGATTGTATAGCAAATTATAACAACCAACTGGATAACAAACA AACAAATCTGATGCTCCCTGAGTCAACTGTTTATGGTGATGTGGACCTTAGTAACAAAATCAATGAGATG AAAACCTTCAATAGCCCAAATCTGAAGGATGGGCGTTTTGTCAATCCATCAGGGCAGCCCACTCCCTACG CCACCACGCAGCTCATCCAGGCAAATCTCATCAACAATATGAATAACGGCGGCGGGGACTCCAGCGAGAA ACACTGGAAGCCTCCGGGGCAGCAGAAACAGGAAGTGGCACCAATTCAGTATAACATCATGGAGCAAAAC AAGTTGAACAAAGATTATCGGGCAAATGACACAATCCTTCCAACCATCCCGTACAACCATTCNTATGACC AGAATACAGGAGGATCCTACAACAGCTCAGATCGGGGCAGTAGTACATCTGGGAGTCAAGGCCACAAGAA AGGGGCACGGACACCAAAGGCACCCAAGCAGGGTGGCATGAACTGGGCAGACTTGCTGCCCCCTCCCCCA GCGCATCCTCCCCCACACAGCAACAGCGAGGAATACAGCATGTCTGTAGATGAAAGCTATGATCAAGAAA TGCCGTGCCCTGTTCCGCCTGCACGGATGTATTTGCAACAGGATGAATTAGAAGAAGAGGAAGCTGAAAG AGGCCCCACGCCCCCTGTCCGGGGGGCAGCTTCGTCCCCAGCTGCTGTGTCCTACAGCCATCAGTCCACG GCCACTCTCACCCCGTCTCCCCAGGAAGAGCTCCAGCCTATGTTACAGGATTGTCCGGAGGACCTCGGCC ACATGCCCCACCCACCAGACAGGAGGCGGCAGCCGGTGAGTCCTCCTCCACCACCACGGCCAATTTCCCC ACCACATACTTACGGCTACATTTCAGGACCCCTGGTCTCAGACATGGATACGGATGCCCCAGAAGAGGAG GAAGACGAAGCTGACATGGAAGTTGCTAAGATGCAGACCCGAAGGCTGTTGTTACGTGGGCTTGAACAGA CCCCAGCATCTAGTGTTGGGGACCTCGAGAGCTCGGTCACTGGCTCCATGATCAATGGCTGGGGCTCAGC CTCAGAAGAGGACAACATTTCCAGTGGGCGCTCCAGTGTCAGTTCATCGGATGGTTCCTTTTTCACTGAC GCGGATTTTGCCCAGGCAGTTGCAGCAGCCGCCGAGTATGCGGGCCTGAAGGTGGCTCGCCGCCAGATGC AGGATGCTGCTGGCCGCCGACACTTCCATGCCTCCCAGTGCCCTAGGCCCACGAGTCCTGTGTCTACAGA CAGCAACATGAGTGCTGCTGTAATCCAGAAAGCCAGACCCACCAAGAAACAGAAGCACCAGCCAGGACAT CTGCGCAGAGAGGCCTACACAGATGATCTTCCACCACCTCCAGTGCCACCGCCTGCTATAAAATCGCCCA GTGTCCAGTCCAAGGCACAGCTGGAGGCACGGCCTATAATGGGGCCAAAGCTCGCTTCTATAGAAGCGAG AGCAGACAGATCATCAGACAGAAAAGGCGGCAGTTACAAGGGGAGAGAAGCTCTGGACGGAAGACAAGTC ACTGACCTGCGAACAAGCCCAGGTGACCCCAGAGAAGCACAGGAGCAGCCAAACGAAGGGAAAGCACGAG GGACGAAGACAGCAAAGCGAGACCTTCCACCAGCGAAGACACACCTCATCCCAGAGGATATCCTTCCGTA CTGTAGGCCTACTTTCCCAACGTCAAACAACCCTAGGGATCCCAGTTCTTCAAGCTCAATGTCATCAAGA GGCTCAGGAAGCCGACAGAGAGAGCAAGCAAATGTGGGCCGAAGAAATATGGCAGAAATGCAAGTACTTG GGGGATTTGAAAGAGGAGATGAGAATAATGAAGAATTAGAGGAAACTGAAAGCTGA
	nt 1 - nt 4956 of AF041082.1
Notes:	The Robo1 probe used in this study by Yuan et al., 1999 [PMID:10433822] was "rat Robo1, 4956-bp fragment containing the entire ROBO1 coding region (Kidd et al., 1998 [PMID:9458045] )".
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	Swiss Webster
Age:	10.5 dpc
Theiler Stage:	TS17
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Fixation:	4% paraformaldehyde
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Yuan W; Zhou L; Chen JH; Wu JY; Rao Y; Ornitz DM, 1999 [PMID:10433822] . Indexed and spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1006/dbio.1999.9371] [ PMID:10433822] Yuan W, Zhou L, Chen JH, Wu JY, Rao Y, Ornitz DM 1999 The mouse SLIT family: secreted ligands for ROBO expressed in patterns that suggest a role in morphogenesis and axon guidance. Dev Biol (212):290-306
	[ doi:10.1016/S0092-8674(00)80915-0] [ PMID:9458045] Kidd T, Brose K, Mitchell KJ, Fetter RD, Tessier-Lavigne M, Goodman CS, Tear G 1998 Roundabout controls axon crossing of the CNS midline and defines a novel subfamily of evolutionarily conserved guidance receptors. Cell (92):205-15
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source