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EMAGE:4928

Rock2 Rho-associated coiled-coil containing protein kinase 2 ( MGI:107926)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4928
Fig 1. [doi:10.1002/dvdy.10212] Copyright: This image is from Zhao Z; Rivkees SA, "Rho-associated kinases play an essential role in cardiac morphogenesis and cardiomyocyte proliferation." Dev Dyn 2003 226: 24-32. Reprinted with permission of Wiley-Liss Inc. [PMID:12508221] .

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: a, atrium; hl, hindlimb bud; fl, forelimb bud; md, mandibular arch; rv, right ventricle. Scale bar = 500 um. The authors state that Rock1 also has a similar expression in the heart to that of Rock2 as shown here.
Expression Pattern Description
Spatial Annotation:
EMAGE:4928Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4928_wholemount_moderate_3D_1.wlz
4928_wholemount_notDetected_3D_1.wlz
4928_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4928_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
left auricular region cardiac muscle
detected detected
common atrial chamber left part cardiac muscle
detected detected
common atrial chamber right part cardiac muscle
detected detected
right auricular region cardiac muscle
detected detected
primitive ventricle cardiac muscle
detected detected
outflow tract
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Rock2 probeA
Entity Detected:Rock2, Rho-associated coiled-coil containing protein kinase 2 ( MGI:107926)
Notes:The Rock2 probe template used in this study by Zhao & Rivkees, 2003 [PMID:12508221] was "700 bp" and was "obtained by polymerase chain reaction from embryonic cDNA".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6J
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Zhao Z & Rivkees SA, 2003 [PMID:12508221] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/dvdy.10212] [ PMID:12508221] Zhao Z, Rivkees SA 2003 Rho-associated kinases play an essential role in cardiac morphogenesis and cardiomyocyte proliferation. Dev Dyn (226):24-32
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE