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EMAGE:4929

Rpl10 ribosomal protein 10 ( MGI:105943)
TS18 (9.5 dpc)
immunohistochemistry

Data Images
EMAGE:4929
Fig 1B. [doi:10.1046/j.1432-0436.1999.6430161.x] Mills AA; Mills MJ; Gardiner DM; Bryant SV; Stanbridge EJ, "Analysis of the pattern of QM expression during mouse development." Differentiation 1999 Mar;64(3):161-71. [PMID:10234813]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4929Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4929_wholemount_moderate_3D_1.wlz
4929_wholemount_weak_3D_1.wlz
4929_wholemount_notDetected_3D_1.wlz
4929_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4929_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
surface ectoderm
detected detected
regionalExpression on the surface of the embryo; more restricted compared to that seen at E8.5.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:3719812
Entity Detected:Rpl10, ribosomal protein 10 ( MGI:105943)
Antigen:sense strand is shown

>MGI:3719812
VKYIPSRGPLDKWRALHS
aa 197 - aa 214 of NP_006004.1
Notes:The anti-Rpl10 (QM) antibody used in this study by Mills et al., 1999 [PMID:10234813] was "QM C-17 polyclonal antibody (Santa Cruz Biotechnology, 100 ug/0.1 ml)". Editors Note: the datasheet from Santa Cruz Biotech states that "QM (C-17) is an affinity purified rabbit polyclonal antibody raised against a peptide mapping at the C-terminus of QM of human origin (differs from corresponding mouse sequence by a single amino acid)."
Antibody Type:polyclonal
Raised In:rabbit
Supplier:Santa Cruz Biotechnology
Catalogue Number:sc-798
Specimen
Organism:mouse
Strain:Swiss
Age:9.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Secondary Antibody:goat anti-rabbit
Labelled with:horse radish peroxidase
Visualisation method:DAB
General Information
Authors:Mills AA; Mills MJ; Gardiner DM; Bryant SV; Stanbridge EJ, 1999 [PMID:10234813] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1046/j.1432-0436.1999.6430161.x] [ PMID:10234813] Mills AA, Mills MJ, Gardiner DM, Bryant SV, Stanbridge EJ 1999 Analysis of the pattern of QM expression during mouse development. Differentiation (64):161-71
Links:MGI:3719821 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI