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EMAGE:4935

Runx3 runt related transcription factor 3 ( MGI:102672)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4935 EMAGE:4935
Figure 1A. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2002.0640] Dev Biol 245: 95-108, Stricker S; Fundele R; Vortkamp A; Mundlos S, "Role of Runx genes in chondrocyte differentiation." Copyright 2002 [PMID:11969258] Figure 1B. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2002.0640] Dev Biol 245: 95-108, Stricker S; Fundele R; Vortkamp A; Mundlos S, "Role of Runx genes in chondrocyte differentiation." Copyright 2002 [PMID:11969258]

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4935Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4935_wholemount_possible.wlz
4935_wholemount_notDetected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4935_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
1st branchial arch
not detected not detected
homogeneous
2nd branchial arch
not detected not detected
homogeneous
forelimb bud
not detected not detected
homogeneousNot detected in the region anterior of the limb bud, corresponding to the future shoulder girdle region.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Runx3 probeA
Entity Detected:Runx3, runt related transcription factor 3 ( MGI:102672)
Sequence:sense strand is shown

>Runx3 probeA
CCTACTAAGCACCCTGGAGACTCTCGGGCCAGCCGAATCCTTCCCTCCAGCCCGAGACTACAAGAAGAAA
CAGACTTTGGCCTGGTCCCAGGGGCCAGAGCTGGCAGTGGCTCCCAGAGCTCTGTCCAGCCACAAGTTTG
AGCAGAGGATGTGGGGACCTCCCACAGGACACTGCTCCAACCCAAATCAATGTCCTCTCTAGCTGTGCAT
CCCAAACCCACCTCTTGTCTCCAGCCATGCCCAGGATCAATTCTAGAATCAGAGGCTACCCCTGCCTGTG
TGGGGAGGGCCTTTGCTGGCAAGAGAAGCTCAGGCTCAGAGATGTCTGGACTT
nt 1499 - nt 1831 of NM_019732.1
Notes:The Runx3 probe template used in this study by Stricker et al., 2002 [PMID:11969258] was "generated from 3' untranslated sequences showing minimal or no homology to the other Runx genes", "using primers F: CTACTAAGCACCCTGGACAC and R: GAAAGTCCAGACATCTCTGAG".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Stricker S; Fundele R; Vortkamp A; Mundlos S, 2002 [PMID:11969258] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.2002.0640] [ PMID:11969258] Stricker S, Fundele R, Vortkamp A, Mundlos S 2002 Role of Runx genes in chondrocyte differentiation. Dev Biol (245):95-108
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE