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EMAGE:4936

Runx2 runt related transcription factor 2 ( MGI:99829)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4936 EMAGE:4936
Figure 1A-Runx2-10.5. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2002.0640] Dev Biol 245: 95-108, Stricker S; Fundele R; Vortkamp A; Mundlos S, "Role of Runx genes in chondrocyte differentiation." Copyright 2002 [PMID:11969258] Figure 1B-Runx2-10.5. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2002.0640] Dev Biol 245: 95-108, Stricker S; Fundele R; Vortkamp A; Mundlos S, "Role of Runx genes in chondrocyte differentiation." Copyright 2002 [PMID:11969258]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Arrows point to expression in the furture shoulder girdle.
Expression Pattern Description
Spatial Annotation:
EMAGE:4936Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4936_wholemount_moderate.wlz
4936_wholemount_possible.wlz
4936_wholemount_notDetected.wlz
4936_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4936_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
forelimb bud
detected detected
regionalRunx2 is expressed in a region anterior to the forelimb bud, corresponding to the future shoulder girdle region.
branchial arch
detected detected
regionalExpressed in some cells of the 1st and 2nd branchial arches.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Runx2 probeB
Entity Detected:Runx2, runt related transcription factor 2 ( MGI:99829)
Notes:The Runx2 probe template used in this study by Stricker et al., 2002 [PMID:11969258] was "generated from 3' untranslated sequences showing minimal or no homology to the other Runx genes", "using primers F: GTGTTCTGTGGTCTCTGAG and R: GGCAAAAGCTTGCAGAACTC". Editors note: the full 3'UTR sequences of mouse Runx2 are under-represented in the public sequence databases.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Stricker S; Fundele R; Vortkamp A; Mundlos S, 2002 [PMID:11969258] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.2002.0640] [ PMID:11969258] Stricker S, Fundele R, Vortkamp A, Mundlos S 2002 Role of Runx genes in chondrocyte differentiation. Dev Biol (245):95-108
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE