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EMAGE:4942

Gpc1 glypican 1 ( MGI:1194891)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4942
Fig4(wm)Gpc-1. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.bbrc.2006.10.185] Biochem Biophys Res Commun 352: 55-60, Luxardi G; Galli A; Forlani S; Lawson K; Maina F; Dono R , Glypicans are differentially expressed during patterning and neurogenesis of early mouse brain. Copyright 2007

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: limb buds (lb), first and second branchial arches (I and II), somites (s), forebrain (fb), midbrain (mb), hindbrain (hb), heart (h).
Expression Pattern Description
Spatial Annotation:
EMAGE:4942Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4942_wholemount_moderate_3D_1.wlz
4942_wholemount_weak_3D_1.wlz
4942_wholemount_notDetected_3D_1.wlz
4942_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4942_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
limb
detected detected
Gpc-1 is also expressed in the limb buds.
trunk somite
detected detected
tail somite
detected detected
1st branchial arch maxillary component
detected detected
Gpc-1 is expressed in the mandibular and maxillary processes.
1st branchial arch mandibular component
detected detected
Gpc-1 is expressed in the mandibular and maxillary processes.
2nd branchial arch
detected detected
telencephalon
detected detected
regionalGpc-1 becomes highest in the developing dorso-anterior telencephalon and ventral telencephalic ganglionic eminences. Gpc-1 expression is seen in the telencephalic neuroepithelium.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Gpc1 probeA
Entity Detected:Gpc1, glypican 1 ( MGI:1194891)
Sequence:sense strand is shown

>Gpc1 probeA
GGAGAGGCTCAGGGCACCACTTGGAATCTGGTTTCCTCCTATGGCCTCGAGACCTGGGGGCACCTGGCTT
ACCAAGGCTGTCAAGGCTCCATCTTCCCTTCCAGGGCCTAGGGGATGCTGAGTTGCTATATCCAGGAAGG
CAGTGGTCCTGACATGCAGAAGGCCTGGAAAAAAGGCTGCTTACCGCTTTTTTTTTTTTTTAATTTTAAT
TTTTTTTTTTAACTTTCTTTTAGTCACTGGGTTAGAGAGGCTATTGCCAGCACAGCACCAAGGCCTGCCC
AGTAGTCAGGTCCCCTGGTCCAGGATGGGTATTCTCCATAATTGGTCTGTGGGTTTGTTGGGCCCATGCC
CTGAGCACACTCAACCCAGCCACAGCCCTGCTAGTGCCACTCAAGCAGGGGCACTGGGAGCTAGACCTGG
GCTCTGAGATGAGTGCCTAGGTGTCAGGACAGCAGCACCCATGCTGTACAGGGCAGCCGGGACAGTGGCC
AGGGTACTGGGT
Notes:The Gpc1 probe used in this study by Luxardi et al., 2007 [PMID:17107664] is described as follows: a Gpc-1clone was identified by BLAST search of GenBank EST database using cDNA sequences of the NCBI database (gpc-1: Accession No. AF185613). A corresponding EST clone was obtained from RZPD Consortium (www.rzpd.de/cgi-bin/db). Gpc-1: RZPD clone ID-IMAGE480151. ESTs clones were confirmed by restriction enzyme digestion and sequence analysis. Digoxygenin RNA probes were synthesized according to the manufacturer's instruction (Roche).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Luxardi G; Galli A; Forlani S; Lawson K; Maina F; Dono R, 2007 [PMID:17107664] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.bbrc.2006.10.185] [ PMID:17107664] Luxardi G, Galli A, Forlani S, Lawson K, Maina F, Dono R 2007 Glypicans are differentially expressed during patterning and neurogenesis of early mouse brain. Biochem Biophys Res Commun (352):55-60
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE