EMAGE mouse gene expression spatial database

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EMAGE:4945

Gpc4 glypican 4 ( MGI:104902)
TS17 (10.5 dpc)
in situ hybridisation

Data Images


Fig4(wm)Gpc-4. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.bbrc.2006.10.185] Biochem Biophys Res Commun 352: 55-60, Luxardi G; Galli A; Forlani S; Lawson K; Maina F; Dono R , Glypicans are differentially expressed during patterning and neurogenesis of early mouse brain. Copyright 2007

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Image annotations: limb buds (lb), first and second branchial arches (I and II), somites (s), forebrain (fb), midbrain (mb), hindbrain (hb), heart (h).

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

4945_wholemount_moderate_3D_1.wlz

4945_wholemount_weak_3D_1.wlz

4945_wholemount_notDetected_3D_1.wlz

4945_wholemount_strong_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:4945_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

midbrain

detected

brain

detected

regional

Gpc-4 expression is described in hypothalamus and adjacent to the optic stalk.

1st branchial arch maxillary component

detected

1st branchial arch mandibular component

detected

2nd branchial arch

detected

regional

Gpc-4 is expressed in the mandibular and maxillary processes.

limb

detected

trunk somite

detected

tail somite

detected

telencephalon

detected

Gpc-4 is detected in the developing telencephalic neuroepithelium.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Gpc4 probeA
Entity Detected:	Gpc4, glypican 4 ( MGI:104902)
Sequence:	sense strand is shown >Gpc4 probeA CTGCTTTCCATCGGGTCTCATTCTGTCCCCTAAAGGTGGGAGCGCGTCCGTTAGGGTCCGCACCATGGCA CGCTTAGGCTTGCTCGCGCTCCTCTGCACCCTGGCCGCGCTCAGCGCCTCGCTGCTGGCTGCGGAGCTCA AGTCGAAAAGTTGCTCGGAAGTGCGACGTCTCTACGTGTCCAAAGGCTTCAACAAGAATGATGCCCCCCT CTATGAGATCAACGGTGACCATTTGAAGATCTGTCCCCAGGATTATACATGCTGTTCTCAAGAGATGGAA GAGAAGTACAGCCTGCAAAGTAAAGATGATTTCAAAACCGTGGTCAGCGAACAGTGCAACCATTTGCAAG CCATCTTTGCATCCCGTTACAAGAAGTTTGATGAATTCTTCAAAGAACTTCTTGAAAATGCAGAGAAATC CCTGAATGATATGTTCGTGAAGACATATGGCCACTTATACATGCAAAATTCAGAGCTATTTAAAGATCTC TTCGTTGAGTTGAAGCGCTACTATGTGGCGGGAAATGTGAACCTGGAAGAAATGTTAAATGACTTCTGGG CTCGCCTTCTGGAGCGCATGTTTCGCCTGGTGAACTCCCAGTACCATTTTACAGATGAGTACTTGGAATG TGTGAGCAAATATACAGAGCAGCTGAAGCCTTTTGGAGATGTCCCTCGGAAACTGAAGCTCCAGGTTACC CGCGCATTTGTTGCAGCCAGGACCTTCGCTCAAGGCTTAGCAGTTGCAAGGGATGTAGTAAGCAAAGTGT CCGTGGTGAACCCCACAGCTCAGTGCACCCATGCACTGCTGAAGATGATCTACTGCTCCCACTGCCGGGG CCTGGTGACTGTGAAGCCCTGTTACAACTATTGCTCAAACATCATGCGAGGCTGTTTGGCCAACCAAGGA GACCTTGATTTTGAGTGGAACAATTTCATAGATGCTATGCTGATGGTGGCAGAGAGGCTGGAGGGTCCTT TCAACATTGAGTCCGTTATGGATCCCATCGATGTGAAGATTTCTGATGCTATCATGAATATGCAGGATAA TAGTGTTCAAGTGTCTCAGAAGGTTTTCCAAGGCTGTGGCCCTCCGAAGCCTCTCCCAGCTGGACGAATT TCTCGCTCCATCTCTGAAAGTGCCTTCAGTGCTCGATTCAGACCTTATCATCCAGAGCAACGCCCAACCA CGGCAGCTGGCACTAGTTTGGACCGACTGGTTACTGATGTCAAGGAGAAACTGAAACAAGCTAAGAAGTT CTGGTCCTCTCTCCCAAGCACCGTTTGCAATGATGAGAGGATGGCAGCAGGAAATGAAAATGAGGATGAC TGCTGGAATGGCAAAGGCAAAAGCAGGTACCTGTTTGCAGTGACAGGAAATGGATTGGCCAACCAGGGCA ACAACCCAGAAGTCCAGGTTGACACCAGCAAGCCAGACATACTGATCCTTCGTCAGATCATGGCCCTTCG GGTTATGACCAGTAAAATGAAGAATGCTTACAATGGAAATGACGTGGACTTCTTTGACATCAGTGATGAG AGTAGTGGAGAGGGAAGTGGAAGCGGATGTGAATATCAGCAGTGCCCTTCGGAGTTCGAGTACAACGCCA CTGACCATTCTGGGAAGAGTGCCAACGAGAAAGCTGAC
	nt 406 - nt 2053 of NM_008150.1
Notes:	The Gpc4 probe template used in this study by Luxardi et al., 2007 [PMID:17107664] is described as follows: Gpc-4 cDNA was cloned by RT-PCR of mouse embryonic kidney. Gpc-4 F (5' CTGCTTTCCATCGGGTCTCATTCTG 3' ) and R (5' GTCAGCTTTCTCGTTGGCACTCTTCC 3' ). PCR products were cloned in pGEM-T-EasyVector (Promega).
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	CD-1
Age:	10.5 dpc
Theiler Stage:	TS17
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

General Information

Authors:	Luxardi G; Galli A; Forlani S; Lawson K; Maina F; Dono R, 2007 [PMID:17107664] , Indexed and Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/j.bbrc.2006.10.185] [ PMID:17107664] Luxardi G, Galli A, Forlani S, Lawson K, Maina F, Dono R 2007 Glypicans are differentially expressed during patterning and neurogenesis of early mouse brain. Biochem Biophys Res Commun (352):55-60
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source