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EMAGE:4961

Ptch2 patched homolog 2 ( MGI:1095405)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:4961
Fig4E. [doi:10.1016/S0006-291X(03)00183-9] Reprinted from Biochem Biophys Res Comm, vol 302 Ohuchi H; Tao H; Ohata K; Itoh N; Kato S; Noji S; Ono K, "Fibroblast growth factor 10 is required for proper development of the mouse whiskers." pp 562-7. Copyright (1997) with permission from Elsevier. [PMID:12615071]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4961Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4961_wholemount_possible.wlz
4961_wholemount_notDetected.wlz
4961_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4961_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
1st branchial arch
detected detected
regionalExpressed in maxilla in two spots of whisker placode.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Ptch2 probeA
Entity Detected:Ptch2, patched homolog 2 ( MGI:1095405)
Notes:The Ptch2 (Ptc2) probe used in this study by Ohuchi et al., 2003 [PMID:12615071] is indicated as that described by Motoyama et al, 1998 [PMID:9858693] , who in turn refer to Motoyama et al, 1998 [PMID:9462734] , who refer to Takabatake et al, 1997 [PMID:9237688] . Editors Note: Takabatake et al., did not perform in situ hybridisations, however they did isolate a partial 766nt mouse ptc2 sequence (AB000847). It is unclear from the description if this region was used as probe template in the current study.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Ohuchi H; Tao H; Ohata K; Itoh N; Kato S; Noji S; Ono K, 2003 [PMID:12615071] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0006-291X(03)00183-9] [ PMID:12615071] Ohuchi H, Tao H, Ohata K, Itoh N, Kato S, Noji S, Ono K 2003 Fibroblast growth factor 10 is required for proper development of the mouse whiskers. Biochem Biophys Res Commun (302):562-7
 [ doi:10.1016/S0925-4773(98)00149-X] [ PMID:9858693] Motoyama J, Heng H, Crackower MA, Takabatake T, Takeshima K, Tsui LC, Hui C 1998 Overlapping and non-overlapping Ptch2 expression with Shh during mouse embryogenesis. Mech Dev (78):81-4
 [ doi:10.1038/ng0298-104] [ PMID:9462734] Motoyama J, Takabatake T, Takeshima K, Hui C 1998 Ptch2, a second mouse Patched gene is co-expressed with Sonic hedgehog. Nat Genet (18):104-6
 [ doi:10.1016/S0014-5793(97)00645-5] [ PMID:9237688] Takabatake T, Ogawa M, Takahashi TC, Mizuno M, Okamoto M, Takeshima K 1997 Hedgehog and patched gene expression in adult ocular tissues. FEBS Lett (410):485-9
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE